April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Tear Cytokines in Non-Dry Eye and Dry Eye Participants After Exposure to a Low Humidity Environmental Exposure Chamber
Author Affiliations & Notes
  • Lakshman N Subbaraman
    CCLR, School of Optometry, University of Waterloo, Waterloo, ON, Canada
  • David J McCanna
    CCLR, School of Optometry, University of Waterloo, Waterloo, ON, Canada
  • Holly Irene Lorentz
    Inflamax Research Inc, Mississauga, ON, Canada
  • Fiona Soong
    Inflamax Research Inc, Mississauga, ON, Canada
  • Anne Marie Salapatek
    Inflamax Research Inc, Mississauga, ON, Canada
  • Lyndon William Jones
    CCLR, School of Optometry, University of Waterloo, Waterloo, ON, Canada
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3682. doi:
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      Lakshman N Subbaraman, David J McCanna, Holly Irene Lorentz, Fiona Soong, Anne Marie Salapatek, Lyndon William Jones; Tear Cytokines in Non-Dry Eye and Dry Eye Participants After Exposure to a Low Humidity Environmental Exposure Chamber. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3682.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To quantify tear cytokine levels in Non-Dry eye (NDE) and Dry Eye (DE) participants before and after exposure to a Low Humidity-Environmental Exposure Chamber (LH-EEC).

 
Methods
 

Basal tear samples were collected (pre-exposure) from 8 NDE and 8 DE participants prior to their entry into the LH-EEC. The participants remained in the LH-EEC with controlled temperature (23±3°C), relative humidity (10±3%) and air velocity (3-5ft/sec) and performed visual tasking for 180 minutes. Prior to their exit, basal tears were collected (post-exposure). Subsequently, participants remained in the clinic and tears were collected again after 60 minutes (recovery). The collected tear samples were analyzed using the human pro-inflammatory V-plex assay (IFN-γ, IL-10, IL-12 p70, IL-13, IL-1β, IL-2, IL-4, IL-6, IL-8 and TNF-α) using the MesoScale Discovery Platform.

 
Results
 

There was a significant difference between the NDE and DE participants for IL-2 (p=0.009), however, no significant differences were seen between the two groups for other cytokines. The mean and standard deviation for each cytokine in the NDE and DE participants under 3 conditions are provided in the Table. Exposure to the EEC caused over 100% increase in the cytokine concentration in some participants.

 
Conclusions
 

Induction and reduction in tear cytokines vary after exposure to a controlled LH-EEC in NDE and DE participants. The LH-EEC model can be used to understand the role of tear film biomarkers in the pathogenesis of dry eye.

 
 
Tear cytokine levels in NDE and DE participants under three different conditions
 
Tear cytokine levels in NDE and DE participants under three different conditions
 
Keywords: 486 cornea: tears/tear film/dry eye  
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