April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Immunogenicity of the fish scale-derived collagen matrix for corneal reconstruction
Author Affiliations & Notes
  • T Huibertus van Essen
    Ophthalmology, Leiden Univ Medical Center, Leiden, Netherlands
  • Lisanne van Zijl
    Research, Aeon Astron Europe BV, Leiden, Netherlands
  • Aat A Mulder
    Dermatology, Leiden Univ Medical Center, Leiden, Netherlands
  • Sarah J Sparks
    Research, Aeon Astron Europe BV, Leiden, Netherlands
  • Chien C Lin
    Research, Aeon Astron Europe BV, Leiden, Netherlands
  • Horng J Lai
    Research, Aeon Astron Europe BV, Leiden, Netherlands
  • Gregorius P M Luyten
    Ophthalmology, Leiden Univ Medical Center, Leiden, Netherlands
  • Abdoelwaheb El Ghalbzouri
    Dermatology, Leiden Univ Medical Center, Leiden, Netherlands
  • Martine Jager
    Ophthalmology, Leiden Univ Medical Center, Leiden, Netherlands
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3733. doi:
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      T Huibertus van Essen, Lisanne van Zijl, Aat A Mulder, Sarah J Sparks, Chien C Lin, Horng J Lai, Gregorius P M Luyten, Abdoelwaheb El Ghalbzouri, Martine Jager; Immunogenicity of the fish scale-derived collagen matrix for corneal reconstruction. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3733.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

A transparent, naturally-occurring, easily-obtainable fish scale-derived collagen type I matrix (FSCM) has been developed for reconstructing the cornea, demonstrating good cellular repopulation in vitro. Here we investigated the in vivo immunogenicity of this matrix in a non-immune privileged site in rats.

 
Methods
 

Male Fischer 344/DuCrl albino rats were divided into three equal groups, which received a subcutaneous implant of 1. either the FSCM, or 2. a commercial available collagen type I matrix (Ologen®) used in trabeculectomy surgery, or 3. underwent sham surgery. The animals were sacrificed at 7, 42 or 77 days postoperatively. Two additional groups with an FSCM or Ologen implant received a second matrix in the contra-lateral flank at 21 days postoperative and were sacrificed after 7 additional days. Aside standard haematoxylin and eosin staining, immunohistochemistry was performed for each group (n=3) to identify the immune infiltrate with antibodies against granulocytes (MPO), macrophages (ED-1), lymphocytes (CD3), and immunoglobulins (IgG).

 
Results
 

The infiltrate diminished over time from moderate in the acute phase, dominated by granulocytes and macrophages, to minimal in the chronic phase, dominated by lymphocytes. There was no significant difference in the amount of infiltrate between the FSCM and the two control groups (Ologen® and sham) after 7, 42 and 77 days postoperative. There was no indication of sensitization after the second implantation of either the FSCM or Ologen®.

 
Conclusions
 

The fish scale-derived collagen matrix for corneal reconstruction is not eliciting an unwanted immune reaction.

 
 
Haemotoxylin-eosin staining of the FSCM at 77 days follow-up, showing almost complete absence of infiltrating cells
 
Haemotoxylin-eosin staining of the FSCM at 77 days follow-up, showing almost complete absence of infiltrating cells
 
Keywords: 575 keratoprostheses • 557 inflammation • 554 immunohistochemistry  
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