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Masaki Imayasu, Nobuyuki Kanemaki, Akira Meguro, Nobuhisa Mizuki; A Novel Single Nucleotide Polymorphism Associated with Glaucoma in Shiba-Inu and Shih-Tzu Dogs. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3820.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the association between glaucoma pathology in Shiba-Inu and Shih-Tzu dogs and the polymorphisms of glaucoma candidate gene, ADAMTS10.
90 Shiba-Inu dogs and 115 Shih-Tzu dogs were recruited from the Veterinary Teaching Hospital at Azabu University. 48 Shiba-Inu dogs and 53 Shih-Tzu dogs were diagnosed as glaucoma (IOP, more than 25 mmHg) by tonometry. After purification of genomic DNA from peripheral blood from glaucoma and normal dogs, DNA sequences including 56097365 G>A variant in ADAMTS10 gene, reported as a candidate gene allele for glaucoma in Beagles, were analyzed.
We have found a novel glaucoma candidate SNP (56097358 G>A variant) in exon17 of ADAMTS10gene. Statistically significant association was observed for the 56097358 G>A variant (P=0.0398) of ADAMTS10 gene, with a 2.76-fold (95% confidence interval, 1.07-7.15) increased risk of glaucoma in Shiba-Inu dogs as well as Shih-Tzu dogs (P=0.0408, odds ratio=1.69 (0.97-2.93)). There were no significant associations between cases and controls for the 56097365 G>A variant in Shiba-Inu and Shih-Tzu dogs unlike Beagles.
The results showed that a novel SNP (the 56097358 G>A variant) of ADAMTS10 play an important role in the pathology of glaucoma in Shiba-Inu and Shih-Tzu dogs unlike the 56097365 G>A variant. The 56097358 G>A variant may be a breed-specific gene allele for glaucoma in Shiba-Inu and Shih-Tzu dogs. We anticipated that the single nucleotide sequencing data from this study can be used as a genetic testing to determine for the first time with precision whether a dog has glaucoma and to predict whether a dog will develop glaucoma.
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