April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Differentiating vitreous proteomes in idiopathic epiretinal macular membranes using high-performance liquid chromatography coupled to tandem mass spectrometry
Author Affiliations & Notes
  • Hao Wang
    Ophthalmology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China
  • Le Feng
    Ophthalmology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China
  • Fang Wang
    Ophthalmology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China
  • Footnotes
    Commercial Relationships Hao Wang, None; Le Feng, None; Fang Wang, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3828. doi:
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    • Get Citation

      Hao Wang, Le Feng, Fang Wang; Differentiating vitreous proteomes in idiopathic epiretinal macular membranes using high-performance liquid chromatography coupled to tandem mass spectrometry. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3828.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The aim of this study was to compare the protein profile of vitreous humour from patients with idiopathic epiretinal macular membranes with that of vitreous humour from normal human eyes donated for corneal transplant.

 
Methods
 

Vitreous humour from patients with idiopathic epiretinal macular membranes (n=8) and from normal human eyes donated for corneal transplant (n=8) were studied. The comparative proteomic analysis was performed using reversed phase high-performance liquid chromatography (RP-HPLC) coupled to electrospray Ionization tandem mass spectrometry (ESI-MS/MS).

 
Results
 

321 significant differentially expressed proteins (abundance ratio > 1.5, p < 0.05) were identified, including 132 and 189 proteins up- and downregulated in idiopathic epiretinal macular membranes vitreous compared with the control, respectively. Biological pathway analysis revealed 238 proteins involved in 875 biological pathways; among them, the most remarkable pathways differentially represented between idiopathic epiretinal macular membranes and normal vitreous were the platelet degranulation, regulation of biological quality and blood coagulation, hemostasis and response to wounding.

 
Conclusions
 

These data provide insight into the molecular events possibly involved in the pathogenesis of idiopathic epiretinal macular membranes.

 
 
Process used to identify and quantify proteins by LC-ESI-MS/MS.
 
Process used to identify and quantify proteins by LC-ESI-MS/MS.
 
Keywords: 763 vitreous • 663 proteomics • 585 macula/fovea  
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