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Chunpin Lian, Zongyi Li, Hui Lou, Li Wang, Peng Li, Haibin Tian, Lixia Lu, Weiye Li, Guotong Xu; Intervention of ADSC with modified medium on RCS rat. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3991.
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© ARVO (1962-2015); The Authors (2016-present)
Retinal degeneration (RD) is one of major cause of blindness in the world and no effective therapeutic strategies were available nowadays. Stem cell-based therapy holds the promise of curing retinal degenerative process. The protective effect of adipose-derived stem cells (ADSCs) cultured in modified medium has been investigated.
ADSCs (P1) were grown in modified medium and labeled with lentivirus-GFP. The secretion of HGF and PEDF from ADSCs was determined by ELISA kits. Proliferation ability was measured by MTT method and colony-forming unit test. The therapeutic functions of the modified-medium-cultured ADSCs (P4) were confirmed by transplantation into subretinal space of Royal College of Surgeons (RCS) rats at postnatal 3 weeks of age. RCS rats were divided in three equal groups (n=6 per group), including ADSC (DMEM with low glucose), ADSC (modified medium) and untreated control groups. Q-PCR and TUNEL staining were performed to detect neurotrophic factor gene expression and apoptotic photoreceptors. ERG was performed to evaluate the improvement of visual function.
Compared with DMEM-LG medium cultured ADSCs, ADSCs cultured in modified medium possess higher proliferation ability, and show an increased gene and protein expression levels of HGF and PEDF. In the meantime, the inflammation factor IL-6 expression was significantly decreased. After transplantation into the subretinal space of RCS rat, ADSCs cultured in modified medium show an enhanced rescue effects compared to ADSCs cultured in DMEM with low glucose medium, including increased b-wave amplitude, restored retinal nuclear layer thickness, and decreased apoptotic photoreceptors.
The gene expression and proliferation ability can be affected by culture condition. ADSCs in modified medium show a high proliferation ability, neurotrophic factor gene expression level and decreased expression level of inflammation factor IL-6. The enhanced rescue effects in modified-medium-cultured ADSC may due to the stronger paracrine abilities. These data may provide useful clues for further clinical intervention in RD patients.
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