April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Intervention of ADSC with modified medium on RCS rat
Chunpin Lian; Zongyi Li; Hui Lou; Li Wang; Peng Li; Haibin Tian; Lixia Lu; Weiye Li; Guotong Xu
Author Affiliations & Notes
  • Chunpin Lian
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Zongyi Li
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Hui Lou
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Li Wang
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Peng Li
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Haibin Tian
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Lixia Lu
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Weiye Li
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Ophthalmology, Drexel University College of Medicine, Philadelphia, PA
  • Guotong Xu
    Department of Ophthalmology of Shanghai Tenth People`s, Tongji Eye institute, Tongji University School of Medicine(TUSM), Shanghai, China
    Department of Regenerative Medicine and Stem Cell Research Center, Tongji University School of Medicine(TUSM), Shanghai, China
  • Footnotes
    Commercial Relationships Chunpin Lian, None; Zongyi Li, None; Hui Lou, None; Li Wang, None; Peng Li, None; Haibin Tian, None; Lixia Lu, None; Weiye Li, None; Guotong Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 3991. doi:
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      Chunpin Lian, Zongyi Li, Hui Lou, Li Wang, Peng Li, Haibin Tian, Lixia Lu, Weiye Li, Guotong Xu; Intervention of ADSC with modified medium on RCS rat. Invest. Ophthalmol. Vis. Sci. 2014;55(13):3991.

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Abstract

Purpose: Retinal degeneration (RD) is one of major cause of blindness in the world and no effective therapeutic strategies were available nowadays. Stem cell-based therapy holds the promise of curing retinal degenerative process. The protective effect of adipose-derived stem cells (ADSCs) cultured in modified medium has been investigated.

Methods: ADSCs (P1) were grown in modified medium and labeled with lentivirus-GFP. The secretion of HGF and PEDF from ADSCs was determined by ELISA kits. Proliferation ability was measured by MTT method and colony-forming unit test. The therapeutic functions of the modified-medium-cultured ADSCs (P4) were confirmed by transplantation into subretinal space of Royal College of Surgeons (RCS) rats at postnatal 3 weeks of age. RCS rats were divided in three equal groups (n=6 per group), including ADSC (DMEM with low glucose), ADSC (modified medium) and untreated control groups. Q-PCR and TUNEL staining were performed to detect neurotrophic factor gene expression and apoptotic photoreceptors. ERG was performed to evaluate the improvement of visual function.

Results: Compared with DMEM-LG medium cultured ADSCs, ADSCs cultured in modified medium possess higher proliferation ability, and show an increased gene and protein expression levels of HGF and PEDF. In the meantime, the inflammation factor IL-6 expression was significantly decreased. After transplantation into the subretinal space of RCS rat, ADSCs cultured in modified medium show an enhanced rescue effects compared to ADSCs cultured in DMEM with low glucose medium, including increased b-wave amplitude, restored retinal nuclear layer thickness, and decreased apoptotic photoreceptors.

Conclusions: The gene expression and proliferation ability can be affected by culture condition. ADSCs in modified medium show a high proliferation ability, neurotrophic factor gene expression level and decreased expression level of inflammation factor IL-6. The enhanced rescue effects in modified-medium-cultured ADSC may due to the stronger paracrine abilities. These data may provide useful clues for further clinical intervention in RD patients.

Keywords: 721 stem cells • 412 age-related macular degeneration • 695 retinal degenerations: cell biology  
© 2014, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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