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Sang-Joon Lee, Jeong Hoon Heo, Hyun Woong Kim, Yeh-Chan Ahn; Retrobulbar transplantation of mouse Adipose tissue derived stem cells rescues NaIO2 induced Retinal Degeneration Mouse Model. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4000.
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© ARVO (1962-2015); The Authors (2016-present)
We investigate whether retrobulbar transplantation of mouse adipose tissue derived stem cells (mADSC) have a rescue effect on the retinal degeneration of mouse induced by NaIO3.
mADSCs which were harvested and characterized by surface markers (CD 105, CD 73, and CD 90 (≥95 %), and lack expression of CD 45, CD 34, CD 14 or CD 11b, CD 79) were stained by BrdU before transplantation. Retinal degeneration was induced by intraperitoneal injection of NaIO3 (50 mg/mL) on 7 C57BL6 mice. 3 days after the induction, mADSCs (2 x 104 cells/uL) were transplanted on the retrobulbar space of the left eye (treated group) and normal saline was also injected on the retrobulbar space of the right eye (control group). 3 weeks after transplantation, 7 mice were sacrificed and enucleated. The area of photoreceptor outer segment(OS), outer nuclear layer(ONL), and inner nuclear layer(INL) in 10 um length of the retina located within 2 disc diameter from the disc were analyzed in the control group, treated group, and normal control group. Immunohistochemistry of BrdU, GFAP, carbindin, vimentain, CRALBP, SDF-1, and CXCR4 was done.
The areas of OS, ONL, and INL in the normal C5BL6 mice were 31.28 um2, 46.30 um2, and 29.69 um2. The areas of NaIO2 injected mice retina thicknesses which were OS; 2.19 um2, ONL; 5.69 um2, INL 20.66 um2 decreased significantly. In the mADSCs treated group, the areas of OS (8.57 um2), ONL (13.93 um2), and INL (24.97 um2) were significantly different comparing to the control group. The BrdU stain was not observed in the intraocular tissue including retina. The retina of the treated group were stained with SDF-1 staining more than the control group.
The retrobulbar transplantation of mADSCs have rescue effect for the NaIO3 damaged retina, which could imply indirect effect of the transplanted stem cells on the degenerated retina.
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