April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Phase-Sensitive Optical Coherence Tomography for Quantification of Pulse-induced Trabecular Meshwork Movement in ex-vivo Human and Non-human Primate Eyes
Author Affiliations & Notes
  • Sepideh Hariri
    Bioengineering, University of Washington, Seattle, WA
  • Peng Li
    Bioengineering, University of Washington, Seattle, WA
  • Roberto Reif
    Bioengineering, University of Washington, Seattle, WA
  • Murray A Johnstone
    Bioengineering, University of Washington, Seattle, WA
    Ophthalmology, University of Washington, Seattle, WA
  • Ruikang K Wang
    Bioengineering, University of Washington, Seattle, WA
  • Footnotes
    Commercial Relationships Sepideh Hariri, None; Peng Li, None; Roberto Reif, None; Murray Johnstone, University of Washington (P); Ruikang Wang, Carl Zeiss Meditec (P), Optovue inc. (F)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4240. doi:
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    • Get Citation

      Sepideh Hariri, Peng Li, Roberto Reif, Murray A Johnstone, Ruikang K Wang; Phase-Sensitive Optical Coherence Tomography for Quantification of Pulse-induced Trabecular Meshwork Movement in ex-vivo Human and Non-human Primate Eyes. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4240.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

To use phase-sensitive optical coherence tomography (PhS-OCT) for quantitative assessment of dynamic trabecular meshwork (TM) motion in response to various pressure pulses simulating in-vivo blink and ocular saccades.

 
Methods
 

A PhS-OCT system was used for imaging the trabecular meshwork region in ex-vivo human and non-human primate eyes. The system is coupled with a broad bandwidth light source centered at 1340nm (∼7.2 μm axial resolution) and a ∼92 KHz A-line rate InGaAs array sensor. An anterior segment perfusion system and a pulse transducer were used to induce and record pressure pulses in the anterior chamber of mounted cadaver eyes. Various baseline intraocular pressure (IOP), pulse frequency and pulse amplitude values were used to investigate the possible effects of these parameters on the TM dynamic response.

 
Results
 

Our results show that the TM displacement is increased with the increase of pulse amplitude and is decreased as the baseline IOP is increased. No significant difference was observed in the TM displacement between different pulse frequencies. The analysis of the tissue relaxation curve following the pulses showed marked changes in tissue mechanical response under different IOPs. Figure 1A shows the summarized TM displacement acquired from a non-human primate eye under different baseline IOP and pulse amplitudes, showing the limiting effect of increased IOP on TM response to transient pulses. The tissue relaxation response to 10mmHg pulse under 16 and 32 mmHg baseline IOP are shown in Fig1B, depicting the change in two decay rates (time constant 0.46 s vs. 0.35 s for 16 and 32 mmHg respectively, obtained from exponential fitting). Similar results were observed in the TM of human eyes.

 
Conclusions
 

We used a PhS-OCT system to quantify the TM motion in ex-vivo human and non-human primate eyes. Our results show the effectiveness of PhS-OCT for monitoring the functional TM response to changes in IOP, the only treatable glaucoma risk factor. Results from this study could potentially advance our understanding of mechanisms behind abnormal IOP and aid in decision making about management and treatment in glaucoma patients.

 
 
Figure1: extracted TM displacement as a result of induced pressure in a female non-human primate (Macaca Nemestrina) eye (A) and the tissue relaxation curves for 16 (black curve) and 32 mmHg (red curve) IOP.
 
Figure1: extracted TM displacement as a result of induced pressure in a female non-human primate (Macaca Nemestrina) eye (A) and the tissue relaxation curves for 16 (black curve) and 32 mmHg (red curve) IOP.
 
Keywords: 633 outflow: trabecular meshwork • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 568 intraocular pressure  
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