April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Hypoxia induced relaxation of porcine retinal arterioles in vitro depends on nitric oxide and stimulation of EP4-receptors in the perivascular retina
Author Affiliations & Notes
  • Pernille Oversoe Hansen
    Faculty of Health Sciences, University of Aarhus, Aarhus C, Denmark
    Department of Ophthalmology, Aarhus University Hospital, Aarhus C, Denmark
  • Sidse Kringelholt
    Department of Ophthalmology, Aarhus University Hospital, Aarhus C, Denmark
  • Ulf Simonsen
    Department of Biomedicine, University of Aarhus, Aarhus C, Denmark
  • Toke Bek
    Department of Ophthalmology, Aarhus University Hospital, Aarhus C, Denmark
  • Footnotes
    Commercial Relationships Pernille Oversoe Hansen, None; Sidse Kringelholt, None; Ulf Simonsen, None; Toke Bek, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4345. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Pernille Oversoe Hansen, Sidse Kringelholt, Ulf Simonsen, Toke Bek; Hypoxia induced relaxation of porcine retinal arterioles in vitro depends on nitric oxide and stimulation of EP4-receptors in the perivascular retina. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4345.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: Previous studies have shown that the inhibition of nitric oxide synthase (NOS) and cyclooxygenase (COX) can reduce hypoxia induced relaxation of retinal arterioles in vitro. However, the identity of the NOS and COX products involved in the response are unknown.

Methods: Porcine retinal arterioles with preserved perivascular retinal tissue mounted in a myograph were exposed to hypoxia in the presence of one of the following: The general NO synthase inhibitor L-NAME, the selective iNOS inhibitor 1400W, the selective nNOS inhibitor 7-Nitroindazole, the general COX inhibitor Ibuprofen, or antagonist to the FP- (AL 8810), DP- (BW868A), EP1- (SC-19220), EP2- (PF-044189) or EP4-receptors (GW627368X). The experiments were repeated after removal of the perivascular retinal tissue.

Results: Hypoxia induced significant relaxation of retinal arterioles in the presence of perivascular retinal tissue. This relaxation was significantly reduced in the presence of L-NAME, 1400W, Ibuprofen and the EP4-receptor antagonist GW627368X.

Conclusions: Hypoxia induced relaxation of retinal arterioles depends on nitric oxide produced by iNOS and an effect of COX products mediated by EP4-receptors in the perivascular retinal tissue.

Keywords: 436 blood supply • 548 hypoxia • 688 retina  
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×