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Yong Woo Ji, Young Joo Cho, Chul hee Lee, Soon Ho Hong, Dong Yong Chung, Eung Kweon Kim, Hyung Keun Lee; Comparison of Surface Roughness and Bacterial Adhesion between Cosmetic and Conventional Contact Lense. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4671. doi: https://doi.org/.
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© ARVO (1962-2015); The Authors (2016-present)
To compare physical characteristics of cosmetic contact lenses (Cos-CL) and conventional contact lenses (Con-CL) that might affect susceptibility to bacterial adhesion on the CL surface.
Surface characteristics of Cos-CLs and Con-CLs made from the same material by the same manufacturer were measured by atomic force microscopy (AFM) and scanning electron microscopy (SEM). To determine the extent and rate of bacterial adhesion, Cos-CL and Con-CL were immersed in serum-free RPMI media containing Staphylococcus aureus or Pseudomonas aeruginosa. Additionally, the rate of removal of adherent bacteria was evaluated using hand rubbing or immersion in multipurpose disinfecting solutions (MPDS).
The mean surface roughness (Rq: root-mean-square, Rz: peak-to-valley value) measured by AFM was significantly higher for Cos-CL than for Con-CL. At each time point significantly more S. aureus and P. aeruginosa adhered to Cos-CL than to Con-CL, which correlated with the surface roughness of CL. In Cos-CL, bacteria were mainly found on the tinted surface rather than on the non-colored or convex areas. P. aeruginosa attached earlier than S. aureus to all types of CL. However, P. aeruginosa was more easily removed from the surface of CL than S. aureus by either hand rubbing or MPDS soaking.
Increased surface roughness is an important physical factor for bacterial adhesion in Cos-CL, which may explain why rates of bacterial keratitis rates are higher in Cos-CL users in terms of CL physical characteristics.
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