April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Equivalent UVB-induced and NM-induced corneal injuries heal at different speeds over the course of 7 days post exposure
Author Affiliations & Notes
  • Marion K Gordon
    Pharmacology & Toxicology, Rutgers University, Piscataway, NJ
  • Andrea DeSantis Rodrigues
    Pharmacology & Toxicology, Rutgers University, Piscataway, NJ
  • Iris Po
    Pharmacology & Toxicology, Rutgers University, Piscataway, NJ
  • Rita Hahn
    Pharmacology & Toxicology, Rutgers University, Piscataway, NJ
  • Donald Gerecke
    Pharmacology & Toxicology, Rutgers University, Piscataway, NJ
  • Kathy K H Svoboda
    Biomedical Sciences, Texas A&M, Baylor College of Dentistry, Dallas, TX
  • Footnotes
    Commercial Relationships Marion Gordon, None; Andrea DeSantis Rodrigues, None; Iris Po, None; Rita Hahn, None; Donald Gerecke, None; Kathy Svoboda, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4697. doi:
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      Marion K Gordon, Andrea DeSantis Rodrigues, Iris Po, Rita Hahn, Donald Gerecke, Kathy K H Svoboda; Equivalent UVB-induced and NM-induced corneal injuries heal at different speeds over the course of 7 days post exposure. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4697.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: We have observed that UVB corneal injuries heal faster than equivalent nitrogen mustard (NM)-induced wounds. Our overall goal is to elucidate what mechanisms that are involved in the delay of corneal wound healing after mustard injury.

Methods: Rabbit corneal organ cultures were exposed to nitrogen mustard (NM) or UVB at doses that result in 60% epithelial-stromal separation at 24 hr post exposure. Corneal cultures were allowed to heal for 7 days, then were processed for H and E staining, immunofluorescence, and Western blot analysis .

Results: UVB-exposed corneas reestablished epithelial-stromal integrity within 7 days of exposure, but equivalent corneal injury due to NM exposure was only partially healed by 7 days. In areas where the epithelial-stromal junction was reestablished in NM-exposed corneas, the number of fibroblasts residing in the anterior stroma after 7 days was greatly reduced, and repopulation lagged behind that of UVB-treated corneas. Local areas where the epithelial-stromal junction was imperfectly reformed were often observed in NM-exposed corneas, but not in UVB exposed corneas at 7 days post exposure. Immunofluorescence analysis of organ cultured corneas demonstrated that the expression of the furin was still elevated 7 days after NM exposure. Interestingly, ADAMTS4 was expressed in UVB-exposed corneas for 3 days after exposure, while in NM-exposed corneas ADAMTS4 persisted for 5 days after exposure. The expression level of the enzyme was about 5 times greater in NM-exposed than in UVB treated corneas.

Conclusions: The difference in healing time of equivalent corneal injuries induced by NM-exposure versus UVB-exposure may be related to the extended activity of enzymes like furin and ADAMTS4.

Keywords: 482 cornea: epithelium • 765 wound healing • 519 extracellular matrix  
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