April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Effect of blinking on repeatability of measurements of tear meniscus curvature
Author Affiliations & Notes
  • Franco Coronella
    Eye clinic, University of Cagliari, Cagliari, Italy
  • Giovanni M Satta
    Eye clinic, University of Cagliari, Cagliari, Italy
  • Fossarello Maurizio
    Eye clinic, University of Cagliari, Cagliari, Italy
  • Pietro Emanuele Napoli
    Eye clinic, University of Cagliari, Cagliari, Italy
  • Footnotes
    Commercial Relationships Franco Coronella, None; Giovanni Satta, None; Fossarello Maurizio, None; Pietro Napoli, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4855. doi:
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      Franco Coronella, Giovanni M Satta, Fossarello Maurizio, Pietro Emanuele Napoli; Effect of blinking on repeatability of measurements of tear meniscus curvature. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4855.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: To determine by optical coherence tomography (OCT) the influence of blinking on repeated measurements of tear meniscus curvature (TMC).

Methods: Normal human (n = 21) eyes were imaged to get dimensions of the lower tear meniscus (LTM). On the day before the OCT imaging, all enrolled subjects were tested by McMonnies questionnaire, fluorescein tear break-up time (FTBUT), fluorescein staining of ocular surface, Schirmer I test, a slit examination of the lid margins and meibomian glands. All ocular surface images were obtained immediately (method 1) or 2 seconds (method 2) after a blink, and were repeated on the next day. All examinations were conducted in the same conditions of temperature, brightness, humidity and time of the day.

Results: The average TMC was 646.90 ± 513.43 μm and 563.10 ± 24.28 μm, by method 1 and 2, respectively. In the second case, reliability analysis has revealed a good consistency of measurements (ICC=0.99, p<0.01). Conversely, no significant agreement was obtained for measurements by method 1 (ICC=0.4, p=0.39). Comparing the two systems, we did not found a significantly statistical correlation between the results (ICC=0.09, p=0.39).

Conclusions: Blinking plays a crucial role in dynamic distribution human tears and in determining changes of dimensions of tear meniscus. The standardization of OCT imaging of the ocular surface appears to be necessary to aim a significant repeatability of tear meniscus measurements and a better comparability of results.

Keywords: 480 cornea: basic science • 552 imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • 474 conjunctiva  

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