April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Effect of light on photoreceptor degeneration in a mouse model of iodate-induced retinopathy
Author Affiliations & Notes
  • Lars Wagenfeld
    Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Christina Gieseke
    Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Steffen Jung
    Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Gisbert Richard
    Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Udo Bartsch
    Department of Ophthalmology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
  • Footnotes
    Commercial Relationships Lars Wagenfeld, None; Christina Gieseke, None; Steffen Jung, None; Gisbert Richard, None; Udo Bartsch, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4896. doi:
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      Lars Wagenfeld, Christina Gieseke, Steffen Jung, Gisbert Richard, Udo Bartsch; Effect of light on photoreceptor degeneration in a mouse model of iodate-induced retinopathy. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4896.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Iodate intoxication can lead to a severe damage of the retinal pigment epithelium (RPE). This damage is more pronounced at the posterior pole. This can be seen in animals and in accidental intoxication in humans. It is therefore hypothesized that light amplifies the toxic effect of iodate at the RPE.

Methods: Adult C57BL/6J wild-type mice were injected with sodium iodate (20 mg/kg body weight) or the equivalent volume of saline solution. The animals were kept either in total darkness or in a 12-h dark/12-h light cycle with exposure to full-spectrum light at illumination levels of 4000-5000 lux. After 20 days the cell count of photoreceptors, morphology and dissemination of damage of RPE were compared between the groups at various areas of the retina (25%, 50% and 75% of the distance between optic disc and ora serrata).

Results: A significant reduction of photoreceptors was found in both iodate groups compared to the control [25%: 224 ± 1.9 vs. 136 ± 3.2 in darkness (P=0.001) vs. 101 ± 2.3 in light (P<0.001); 50%: 211 ± 0.8 vs. 141 ± 2 in darkness (P<0.001) vs. 106 ± 2.4 in light (P<0.001); 75%: 204 ± 1.6 vs. 128 ± 2.7 in light (P<0.001) vs. 113 ± 1.4 in darkness (P<0.001)]. A difference between both iodate groups was found at the 50% area (P=0.04).

Conclusions: In our experiments the iodate-induced photoreceptor damage in mice was aggravated by light in certain areas of the retina.

Keywords: 701 retinal pigment epithelium • 670 radiation damage: light/UV • 648 photoreceptors  
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