April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Capsulotomy worsens diabetic retinopathy in the Ins2Akitamice
Author Affiliations & Notes
  • Jose Manuel Romero del Hombre
    Centre for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom
  • Mei Chen
    Centre for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom
  • Gareth Moore
    Centre for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom
  • Heping Xu
    Centre for Experimental Medicine, Queen's University Belfast, Belfast, United Kingdom
  • Footnotes
    Commercial Relationships Jose Manuel Romero del Hombre, None; Mei Chen, None; Gareth Moore, None; Heping Xu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 4906. doi:
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    • Get Citation

      Jose Manuel Romero del Hombre, Mei Chen, Gareth Moore, Heping Xu; Capsulotomy worsens diabetic retinopathy in the Ins2Akitamice. Invest. Ophthalmol. Vis. Sci. 2014;55(13):4906.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Cataract surgery increases the risk of developing diabetic retinopathy (DR) and accelerates the progression of pre-existing DR. Recent evidence suggests that cataract surgery elicits retinal pro-inflammatory gene expression, although the underlying pathogenic mechanisms remain ill-defined. In this study, we investigated the effect of capsulotomy on visual function, retinal immune cell activation and photoreceptor stress in the Ins2Akita mice, a mouse model of Type-1 diabetes.

Methods: Male heterozygous Ins2Akita mice (2 months of hyperglycemia) and C57BL/6J age-matched siblings were used in this study. An incision (1mm) was made in the peripheral cornea and Capsulotomy was performed in the anterior lens capsule of the right eye. Control mice received corneal incision without capsulotomy in the right eye. The unoperated left eyes were used as internal controls. Forty days following surgery, retinal function was assessed by electroretinography (ERG). Neuronal retinal damage and microglial activation were assessed by imunohistochemistry.

Results: The Ins2Akita mice receiving capsulotomy presented lower scotopic a-wave, b-wave and oscillatory potentials amplitudes compared to other experimental groups. Fundus images, SD-OCT and H&E staining did not show significant changes between different groups. Immunostaining of Iba-1 and CD68 revealed exacerbated microglial activation and giant cell immune cell infiltration in eyes receiving capsulotomy in Ins2Akita mice. This was accompanied by a disruption of cone photoreceptor outer segments and abnormal rhodopsin expression at the outer nuclear layer.

Conclusions: Our results suggest that capsulotomy induces retinal microglial activation and worsens retinal neuropathy in diabetic eyes.

Keywords: 499 diabetic retinopathy • 445 cataract • 554 immunohistochemistry  
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