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Jie Zhang, Kenichi Saito, Qiang Yang, Koji Nozato, Kei Suzuki, Jennifer J Hunter, David R Williams, Ethan A Rossi; An integrated adaptive optics scanning light ophthalmoscope (AOSLO) and wide-field SLO (WF-SLO) for steerable high resolution retinal imaging. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5017.
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Most state-of-the-art AOSLOs suffer from two major limitations that prevent wide-scale clinical adoption: small field of view (FOV) and reliance on patient fixation for targeting a retinal location. The small FOV makes obtaining images across a large retinal area difficult. Reliance on patient fixation makes targeting specific regions of interest inefficient and often inaccurate, particularly in patients with poor vision. Here we report a novel ophthalmic imaging device designed to solve both of these problems without compromising resolution.
We designed and built a reflective AOSLO (FOV: 1.5°×1.5°) and a refractive point-scan WF-SLO (FOV: 27°×23°) optically and electronically integrated for rapid, simultaneous data acquisition. Inspired by the system presented by Burns et al, we used a steering mirror located at a pupil conjugate plane to allow the AO imaging field to be shifted across 15°. The AOSLO sub-system was designed for near diffraction-limited performance across all steering angles. We tested system performance by acquiring images on five normal eyes.
The WF-SLO, featuring a 15 μm lateral resolution, provided high contrast retinal images in which the optic disk and vasculature were clearly visible. The AOSLO subsystem has a focus range of -6.5D to 1.5D with ~2 μm of lateral resolution. The system was designed for a theoretical uncorrected wavefront error at 790 nm of <1.12 waves (peak-to-valley) and <0.24 waves (RMS) across the entire 15° steering field. We were able to resolve rods and the entire foveal cone mosaic in several eyes.
This new instrument overcomes two of the major limitations of AOSLO imaging. We were able to add this functionality without compromising resolution as evidenced by our ability to resolve both rods and foveal cones. This device has the potential to permit the rapid acquisition of large retinal areas and allow precise targeting of retinal pathology. This will greatly increase the efficiency of clinical imaging by: 1) reducing the need for large overlap between adjacent AOSLO fields, 2) shortening imaging session duration, 3) minimizing light exposure and 4) improving our ability to target previously imaged areas for longitudinal studies.
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