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Xiaoyan Chen, Jiao Wang, Lihua Wang, Dan-Ning Hu, Dongsheng Yan; MicroRNA-135a suppresses Uveal Melanoma Cell Proliferation and Migration. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5062.
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MicroRNAs (miRNAs) can act as either oncogenes or tumor suppressors in tumorigenesis. Evidence indicates that some miRNAs are essential for uveal melanoma cell proliferation and migration. Nevertheless, the role of miR-135a in uveal melanoma remains unknown. Here, we investigated the function of miR-135a in uveal melanoma cells.
Realtime RT-PCR was carried out to detect the expression of miR-135a in uveal melanoma specimens as well as normal controls. Transfection of miR-135a into uveal melanoma cells was performed by Lipofectamine RNAiMAX reagent. The proliferation of uveal melanoma cells was examined by MTS assay. Cell cycle was analyzed by flow cytometry. Cell migration was examined by transwell migration assay.
miR-135a was downregulated in uveal melanoma specimens as compared with normal controls. Transfection of miR-135a into uveal melanoma cells remarkably inhibited cell proliferation and blocked cell cycle at G1 phase. Furthermore, miR-135a suppressed cell migration.
Our results demonstrated that miR-135a may act as a tumor suppressor in uveal melanoma cell proliferation and migration.
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