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Joyce Luciana Covre, Babyla Monteiro, Renata Ruoco Loureiro, Paulo Bispo, Acacio A S Lima Filho, Irina Kerkis, Jose Alvaro Pereira Gomes, Lab. CASO - UNIFESP; Cytotoxicity of antibiotics, antifungals and corticosteroids to human immature dental pulp stem cells cultivated ex vivo. Invest. Ophthalmol. Vis. Sci. 2014;55(13):514.
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To evaluate the cytotoxicity of antibiotics, antifungals and corticosteroids to human immature dental pulp stem cells (hIDPSC) cultivated ex vivo.
hIDPSC were cultivated according to Monteiro et al. (2009).The cells were incubated with amphotericin B, ciprofloxacin, chloramphenicol, dexamethasone, streptomycin, gatifloxacin, gentamicin, moxifloxacin, prednisone and tobramycin, at concentrations of 250, 125, 62.5 and 31.25 µg/mL for 4, 8, 12 and 24 hours. The drugs were diluted in BSS, and prepared without the addition of the preservative benzalkonium chloride, which was tested separately. The toxicity was evaluated by MTT assay.
The first tests performed in the hIDPSC showed that after 4h of incubation did not observe morphological changes. After 8, 12, and 24 hours the antibiotics that presented the higher cytotoxicity were moxifloxacin, gatifloxacin and ciprofloxacin at all concentrations. The antibiotic that showed less toxicity were chloramphenicol and gentamicin. As for corticosteroids, dexamethasone and prednisone did not show morphological changes and cell death. Benzalkonium chloride showed high toxicity in all groups, causing 100% of cells death.
The fluoroquinolones tested showed greater toxicity to hIDPSC when compared with other drugs. None of the corticosteroids tested showed high toxicity. Additional experiments are underway to further confirm our preliminary findings.
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