April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
DIFFERENTIAL PROTEIN EXPRESSION IN KERATOCONUS STROMAL FIBROBLAST
Author Affiliations & Notes
  • Magda J Martinez-Neria
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
  • Karla Patricia Lopez
    Cornea and Refractive Surgery, Instituto de Oftalmologia " Conde de Valenciana", Mexico City, Mexico
  • Enrique O Graue
    Cornea and Refractive Surgery, Instituto de Oftalmologia " Conde de Valenciana", Mexico City, Mexico
  • Antonio Bautista-Hernández
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
  • Rosa P Calvillo-Medina
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
  • Alfredo Dominguez
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
  • Mariana Ortiz-Casas
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
  • Victor Manuel Bautista
    Microbiology and Proteomics, Instituto de Oftalmologia "Conde de Valenciana", Mexico City, Mexico
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5157. doi:
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      Magda J Martinez-Neria, Karla Patricia Lopez, Enrique O Graue, Antonio Bautista-Hernández, Rosa P Calvillo-Medina, Alfredo Dominguez, Mariana Ortiz-Casas, Victor Manuel Bautista; DIFFERENTIAL PROTEIN EXPRESSION IN KERATOCONUS STROMAL FIBROBLAST. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5157.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Keratoconus is a corneal disease characterized by a progressive thinning of the cornea, giving rise to a cone-shaped cornea, myopia, and irregular astigmatism. It has been considered the leading cause of corneal transplantation but although its importance, the exact pathologicals mechanisms associated are still unknown. The aim of this study was to analyze differential protein expression in keratoconus stromal fibroblast in comparison to healthy cornea stromal fibroblast.

Methods: Keratoconus and healthy cornea stromal fibroblasts were isolated, cultivated and characterizated by flow cytometry. After the proteins were extracted, purified a 2D electrophoresis were run and protein expression analysis were performed.

Results: There were no differences in morphology of fibroblasts from keratoconus and healthy cornea. They were positive to vimentine marker. The differential protein expression analysis of the keratoconus stromal fibroblast revealed differences in the protein expression profiles. Seven proteins were found downregulated and two proteins upregulated in the keratoconus stromal fibroblast. Nine proteins were only found in the healthy cornea stromal fibroblast and twenty six were only found in the stromal fibroblast from keratoconus. Identification of proteins by mass spectrometry is being performed.

Conclusions: A novel stromal primary culture of keratoconus fibroblasts was obtained and it was studied by proteomic view. Results showed differential protein expression in keratoconus stromal fibroblast in comparison to healthy cornea stromal fibroblasts. It can help us to understand the pathogenesis of keratoconus and may help us to find biomarkers for the prognosis and opportune diagnosis of keratoconus.

Keywords: 574 keratoconus • 663 proteomics • 484 cornea: stroma and keratocytes  
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