April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Both Healthy and Diseased Fibroblasts Maintain Phenotype in 3D Tissue-Engineered RAFT Constructs Suggesting Potential for Safe Clinical Use and Ex Vivo Disease Modeling Respectively
Author Affiliations & Notes
  • Isobel Massie
    Institute of Ophthalmology, UCL, London, United Kingdom
  • Sarah B Dale
    Institute of Ophthalmology, UCL, London, United Kingdom
  • John Kenneth George Dart
    Institute of Ophthalmology, UCL, London, United Kingdom
    Moorfields Eye Hospital NHS Foundation Trust, London, United Kingdom
  • Julie T Daniels
    Institute of Ophthalmology, UCL, London, United Kingdom
  • Footnotes
    Commercial Relationships Isobel Massie, None; Sarah Dale, None; John Dart, None; Julie Daniels, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5163. doi:https://doi.org/
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      Isobel Massie, Sarah B Dale, John Kenneth George Dart, Julie T Daniels; Both Healthy and Diseased Fibroblasts Maintain Phenotype in 3D Tissue-Engineered RAFT Constructs Suggesting Potential for Safe Clinical Use and Ex Vivo Disease Modeling Respectively. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5163. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Limbal epithelial stem cell (LESC) deficiency can lead to blindness. LESC may be transplanted on amnion but this is biologically variable and supply is unreliable. We have previously developed RAFT (a thin collagen construct) and shown that human limbal epithelial cells (hLE) can be expanded on RAFT. RAFT constructs may also be airlifted, enabling stratification of the epithelium, which is further improved by incorporating human limbal fibroblasts (hLF) into RAFT. However, hLF have the potential to activate to a scarring phenotype, incompatible with transplant. The purpose of this study was to determine if normal hLF in RAFT are safe for use.

Methods: Normal hLF RAFTs were compared with RAFTs containing diseased fibroblasts (dFib) (isolated from scarring conjunctiva, ocular mucous membrane pemphigoid (Oc-MMP)). hLE were seeded onto half of each type of RAFT and cultured for 14 days (submerged) ± 7 days airlifting culture (AL). The scarring phenotype of each RAFT (i.e. hLF vs dFib, ±hLE and ±AL) was assessed using immunohistochemistry (α-sma), zymography (MMP-2 and -9), ELISA (MMP-1, TGF-β) and Sircol assay (collagen production).

Results: Normal hLF and dFib displayed different phenotypes in RAFT. Very few normal hLF in RAFT expressed α-sma. However, in dFib RAFTs, α-sma expression was markedly increased, particularly in hLE- RAFTs. MMP-2 and -9 were expressed more highly on dFib cf. normal hLF on AL- RAFTs (no difference +AL). Patterns of MMP expression were similar for normal hLF and dFib RAFTs: MMP-9 was only seen on hLE+ RAFTs; MMP-2 was found in all conditions, but was increased on hLE- RAFTs and also by AL. There were no differences in MMP-1 expression between any conditions. Collagen production was greater from dFib cf. normal hLF RAFTs by ~3-fold (p<0.05 all except hLE-, AL-). Collagen production from normal hLF RAFTs was unaffected by hLE or AL, but collagen production from dFib RAFTs was decreased after AL (p<0.05). TGF-β production between normal hLF and dFib RAFTs did not differ but fell for both following AL (p<0.05).

Conclusions: Normal hLF do not adopt a scarring phenotype in RAFT and so may be safe for transplant. Conversely, dFib maintain a scarring phenotype in RAFT, indicating that RAFT could be utilised as a 3D ex vivo disease model of Oc-MMP.

Keywords: 484 cornea: stroma and keratocytes • 482 cornea: epithelium • 474 conjunctiva  
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