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Young Sam Kwon, Sae Kwang Ku, Dong Gook Lee, Kwang Ho Jang; Effect of carboxymethyl chitosan on the corneal alkali burn injury in dogs. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5165.
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This study was performed to evaluate the effect of topical application of carboxymethyl chitosan (CMC) on corneal alkali burn injury in dogs.
Fifteen healthy adult male Beagle dogs were used and the corneal alkalie burn was induced by applying a filter paper soaked in NaOH on the cornea for 60 seconds Normal saline (control group), 0.3% ofloxacin, and 0.5% CMC eye drops were applied for 10 days after alkali injury. Corneal epithelial defect and neovascularization were evaluated on days 1, 3, 7, and 10. Hematoxylin-eosin, masson’s trichrome and immunohistochemical stainings were performed to evaluate general histopathology and the expression patterns of apoptotic markers (caspase-3 and PARP), proinflammatory cytokines (TNF-α, IL-1β), inflammatory mediators (COX-2, iNOS) and angiogenesis markers (VEGF).
Corneal defect and neovascularization scores were significantly decreased in CMC treated group compared with control group (p<0.05). Cornea and conjunctiva epithelial thicknesses significantly increased in ofloxacin group and CMC group (p<0.05). Histologically, the thickness of damaged corneal and the number of inflammatory cells significantly reduced in ofloxacin and CMC treated cornea (p<0.05). In addition, the expressions of caspase-3, PARP, TNF-α, IL-1β, COX-2, iNOS, and VEGF were significantly inhibited in the ofloxacin and CMC groups. Particularly, CMC significantly decreased the corneal damage and the expressions of the markers as above compared with ofloxacin.
This study suggests that topical application of 0.5% CMC may have a beneficial effect on corneal alkali burn injury in dogs by regeneration of damaged cornea and alleviation of its inflammatory and apoptotic processes.
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