April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Topical nanomicelles for the treatment of intermediate and posterior uveitis:Focus on micelle size
Author Affiliations & Notes
  • Chandramouli Natarajan
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Ravi Vaishya
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Mitan Gokulgandhi
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Sulabh P Patel
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Mukul Minocha
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Ashim K Mitra
    Dep of Pharmacy, Division of Pharmaceutical Sciences, University of Missouri Kansas city, Kansas city, MO
  • Footnotes
    Commercial Relationships Chandramouli Natarajan, None; Ravi Vaishya, None; Mitan Gokulgandhi, None; Sulabh Patel, None; Mukul Minocha, None; Ashim Mitra, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5266. doi:https://doi.org/
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      Chandramouli Natarajan, Ravi Vaishya, Mitan Gokulgandhi, Sulabh P Patel, Mukul Minocha, Ashim K Mitra; Topical nanomicelles for the treatment of intermediate and posterior uveitis:Focus on micelle size. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5266. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: Our primary aim was to develop and characterize topical dexamethasone-loaded polymeric nanomicelles of various mean sizes (size <80nm).We further determined the effect of nanomicelle size on dexamethasone (DEX) transport across the ocular static barriers.

Methods: Low molecular weight diblock co-polymers, PCL-mPEG were synthesized by ring opening polymerization. Polymers were characterized by H1 NMR, IR spectroscopy, gel permeation chromatography, critical micelle concentration (CMC) and cytotoxicity studies in corneal, conjunctival and retinal cell-lines. DEX-loaded nanomicelles were prepared by modified-film hydration method. The optimized formulation was characterized for solubility of DEX, micelle size and PDI, morphology, in vitro release and in vitro transport across conjunctival cell line. The formulation was also subjected to ex vivo transport across excised rabbit sclera to determine influence of micelle size on DEX transport across the static barrier

Results: The optimized nanomicelle formulation exhibited mean size in range of 10-15nm (DEXMA), 25-30nm (DEXMB) and 55-60nm (DEXMC) with unimodel size distribution and low polydispersity. DEX permeability across the excised rabbit sclera for DEXMA, DEXMB, DEXMC and DEX (Control) were found to be 2.65E-06, 3.00E-06, 1.52E-06 and 1.19E-06 cm/sec, respectively

Conclusions: The permeability studies across the sclera indicates that the nanomicelles with average sizes 10nm and 25nm may have potential to deliver steroidal agents to the back of the eye following top

Keywords: 688 retina • 746 uveitis-clinical/animal model • 503 drug toxicity/drug effects  
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