April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Lateral Diffusion Coefficient in CXL with Corneal Channels
Author Affiliations & Notes
  • Rebecca Marian McQuaid
    Physics, University College Dublin, Dublin 4, Ireland
    Ophthalmology, IROC Innocross, Zurich, Switzerland
  • JiaJun Li
    Physics, University College Dublin, Dublin 4, Ireland
  • Michael Mrochen
    Ophthalmology, IROC Innocross, Zurich, Switzerland
  • Brian Vohnsen
    Physics, University College Dublin, Dublin 4, Ireland
  • Footnotes
    Commercial Relationships Rebecca McQuaid, None; JiaJun Li, None; Michael Mrochen, None; Brian Vohnsen, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5535. doi:
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      Rebecca Marian McQuaid, JiaJun Li, Michael Mrochen, Brian Vohnsen, Advanced Optical Imgaing Group; Lateral Diffusion Coefficient in CXL with Corneal Channels. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5535.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: Recent studies involving corneal cross-linking (CXL) have investigated other methods of riboflavin delivery to the cornea such as Iontophoresis, femtosecond laser-created pockets and trans-epithelium CXL. Standard CXL sees riboflavin diffusion to the cornea over a 30 minutes interval. In this study, we investigate a new method of riboflavin delivery to the cornea without removal of the corneal epithelium. The purpose is to gain insight into the temporal dynamics of corneal riboflavin diffusion via surgically-created intra-stromal channels.

Methods: Porcine globes were obtained from the local slaughterhouse 4 hours post mortem and kept at a temperature of 4 degrees Celsius. An incision was made using a cannula needle, followed by insertion of keraring (Mediphacos Ltd) to create a channel in the cornea for riboflavin diffusion. Monitoring of the cornea with a CCD camera facilitates the study of diffusion characteristics in real time.

Results: The results show that riboflavin can be effectively injected into the cornea via the created diffusion channels without removal of the epithelium layer. The recorded videos show characteristic time-scales on the order of seconds to minutes from which a characteristic diffusion coefficient can be derived on the basis of Fick’s 2nd law of diffusion.

Conclusions: This study investigated the distribution of riboflavin diffusion in the cornea through creation of surgically-created channels in post-mortem porcine eyes. This could be an effective method for riboflavin application for CXL without the need to remove the epithelial layer and thus with reduced patient discomfort..

Keywords: 482 cornea: epithelium • 479 cornea: clinical science • 480 cornea: basic science  

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