April 2014
Volume 55, Issue 13
Free
ARVO Annual Meeting Abstract  |   April 2014
Blue Light-induced Oxidative Stress in Human Corneal Epithelial Cells: Protective Effect of Ethanol Extracts from Mixture of Various Medicinal Plants
Author Affiliations & Notes
  • Kyung Chul Yoon
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Zhengri Li
    Department of Ophthalmology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Je Moon Woo
    Department of Ophthalmology, Ulsan University Hosital, Ulsan, Republic of Korea
  • Soo Hyun Kim
    Department of Dermatology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
  • Jee Bum Lee
    Department of Dermatology, Chonnam National University Medical School and Hospital, Gwangju, Republic of Korea
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5538. doi:
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      Kyung Chul Yoon, Zhengri Li, Je Moon Woo, Soo Hyun Kim, Jee Bum Lee; Blue Light-induced Oxidative Stress in Human Corneal Epithelial Cells: Protective Effect of Ethanol Extracts from Mixture of Various Medicinal Plants. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5538.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To investigate the effects of visible light on human corneal epithelial cells and the impact of natural antioxidants on oxidative stress produced by light overexposure.

Methods: Light emitting diode with various wavelengths (410-830nm) was used to irradiate on human corneal epithelial cells, and then cell viability was assessed. Production of reactive oxygen species (ROS) was analyzed using the 2’,7’-dichlorodihydrofluorescein diacetate (DCF-DA). Ethyl alcohol (EtOH) extracts were prepared from the mixture of various medicinal plants. After application of the EtOH extracts, free-radical-scavenging activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. The induction of antioxidant enzymes including heme oxygenase-1 (HO-1), peroxireoxin-1 (Prx-1), catalase (CAT), and superoxide dismutase-2 (SOD-2) and inhibitory capability of ROS by the extracts were evaluated by real time-PCR and DCF-DA in human corneal epithelial cells.

Results: The viability of human corneal epithelial cells was diminished after irradiation of blue light (above 10J at 410nm and 50J at 480nm). ROS production by blue light irradiation increased in a dose dependant manner. EtOH extracts had potent radical scavenging activity. The application of the extracts increased the expression of HO-1, Prx-1 , CAT, and SOD-2 and attenuated ROS production by blue light.

Conclusions: Overexposure of blue light (410-480nm) may have a harmful impact on human corneal epithelial cells compared to other visual light wavelengths. Medicinal plant extracts can have potent protective effects on blue light-induced oxidative stress.

Keywords: 482 cornea: epithelium • 634 oxidation/oxidative or free radical damage • 424 antioxidants  
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