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HAIYAN WANG, Yenifer S Guerra, Genea Edwards, Sanjoy K Bhattacharya; Differences in aqueous humor phospholipid profiles of DBA/2J mice in ocular normotensive and hypertensive state. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5695.
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© ARVO (1962-2015); The Authors (2016-present)
To determine the differential profiles of phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol and their quantitative differences between aqueous humor (AH) derived from normotensive and hypertensive intraocular pressure states of DBA/2J mice.
All animal procedures were performed adhering to ARVO statement of use of animals in vision research. Intraocular pressure (IOP) measurements were carried out using Tonolab. Mice were divided into normotensive (IOP≤ 15 mm of Hg; 5-6 months of age) or hypertensive (IOP= 24-36 mm of Hg; 8-9 months of age) Normotensive and hypertensive state AH were collected from mice (n= 40) and analyzed. Lipid extraction was performed using the Bligh and Dyer method, and the protein concentrations were determined using the PHAST gel and densitometry. The lipids were identified and quantified using appropriate standards with a TSQ Quantum Access Max triple quadrupole mass spectrometer applying class specific lipid identification settings.
The comparative profiles of phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol between normotensive and hypertensive AH showed several species unique to a state of IOP and as well common between states.
The presence or absence of several phospholipids in the normotensive or hypertensive states may contribute to the better understanding of the glaucomas.
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