Purchase this article with an account.
Shinichiro Ishikawa, Akira Hirata, Jo Nakabayashi, Ryo Iwakiri, Satoshi Okinami; Neuroprotective effects of siRNA, targeting Caspase9 on rat retinal damage induced by different time course of transient ischemic injury.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5738.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
We reported the effect of caspase3 siRNA on the retinal ganglion cell (RGC) damage induced by shorter ischemic injury at 2013 ARVO meeting. We investigated the effect of caspase9 siRNA on transient ischemic injury with a shorter ischemic time.
Seven week-old male Wister rats were used. IOP was kept at 110mmHg for 60min or 120min and then brought back to normal. Caspase9 siRNA were injected into the vitreous cavity 24hr prior to transient ischemic injury. RGCs were labeled with Fluoro-Gold retrogradely at 3 days before the injury. Seven days after the injury, flat-mounts of the retina were examined by fluorescence microscopy.
In 60min group, the numbers of RGCs were 794.2(+/-198.3) /mm2. In 120min group, the numbers were 622.6(+/-89.2) /mm2. There was no statistical significance in both groups.
In contrast to caspase3 siRNA, the neuroprotective effect of caspase9 siRNA is not shown at this model.
This PDF is available to Subscribers Only