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Manami Kuze, Hisashi Matsubara, Masahiko Ayaki, Kazuo Tsubota, Mineo Kondo, Takeshi Morita; Effect of age on the electrical response from intrinsically photosensitive retinal ganglion cells. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5761.
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© ARVO (1962-2015); The Authors (2016-present)
We have previously succeeded in recording intrinsically photosensitive ganglion cells (ipRGC) response to light stimuli from human eyes using four-primary illumination system, which modulates stimulus levels to the ipRGC and other cones independently (Fukuda et al. 2010; 2012). The purpose of this study was to investigate the effect of age on the electroretinogram (ERG) from ipRGCs in humans.
We used the four-primary illumination system (stimulus duration, 250 ms) to stimulate ipRGCs independently of other photoreceptors using a silent-substitution technique (Fukuda et al. J Physiol Anthropol. 2012). Three elder subjects (age, 54.6±5.7 years) and five younger subjects (23.0±1.7 years) are recruited. The implicit times were measured from stimulus onset and offset to the positive peaks and the amplitudes were measured from the baseline to the positive peaks.
Two distinct positive peaks were recorded after the onset (on-response) and offset (off-response) for ipRGCs responses in both groups. The implicit times of on- and off-responses were significantly longer in elder group (on-response,97.3±2.2 ms; off-response, 282.3±5.2 ms) than those in younger group (on-response,79.0±6.5 ms; off-response, 279.0±13.4ms; P<0.05). In addition, the amplitudes of on- and off-responses in elder group were significantly lower (on-response,1.5±0.2μV; off-response, 1.5±0.1μV) than those in younger group (on-response, 2.5±1.6μV; off-response,2.9±1.8μV;P<0.05).
These results suggested that the electrical function of ipRGC is significantly influenced by the age in humans.
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