April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
A new and standardized method to sample and analyse vitreous biopsies in unsolved uveitis by the Cellient® automated cell block system
Author Affiliations & Notes
  • Joachim van Calster
    Ophthalmology, University Hospitals Leuven, Leuven, Belgium
  • Rita Van Ginderdeuren
    Ophthalmology, University Hospitals Leuven, Leuven, Belgium
  • Footnotes
    Commercial Relationships Joachim van Calster, Abbott (C), Allergan (C), DORC (C), Novartis (C), Novartis (F); Rita Van Ginderdeuren, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 5797. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Joachim van Calster, Rita Van Ginderdeuren; A new and standardized method to sample and analyse vitreous biopsies in unsolved uveitis by the Cellient® automated cell block system. Invest. Ophthalmol. Vis. Sci. 2014;55(13):5797.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: In this prospective study a universal protocol for sampling and analysing vitreous material was investigated. Vitreous biopsies are difficult to handle because of the paucity of cells and the gelatinous structure of the vitreous. Histopathological analysis of the vitreous is useful in difficult uveitis cases to differentiate uveitis from lymphoma or infection and to define the type of cellular reaction.

Methods: After isolation of vitreous content by a single port biopsy or by a standardised 23G vitrectomy, 170 consecutive vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automated processor starting from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® (contains a mucolyticum) and Preservcyt®. Routine histochemical and immunostainings were evaluated.

Results: In 79 cases (46%) a single port biopsy and in 91 cases (54%) a tree port pars plana vitrectomy was performed. In 94% of the cases, sufficient material was found for establishing the diagnosis. In 18%, a Cytolyt® wash was necessary to prevent clotting of the tubes in the Cellient® due to the viscosity of the sample. In 21% the diagnosis was an acute inflammation (presence of granulocytes), in 32% chronic active inflammation (presence of T-lymphocytes), in 35% low-grade inflammation (presence of CD68 cells, without T-lymphocytes); and in 5% a malignant process. In 9% a granulomatous inflammation was detected, in 5% glial tissue (floaters). In 6% no diagnosis was found.

Conclusions: A standardized protocol for sampling and handling vitreous biopsies, fixing in PreservCyt® and processing by the Cellient® system gives a superior result in morphology, number of cells, and possibility of immuno-histochemical stainings. The diagnosis can be established or confirmed in more than 90% of cases.

Keywords: 746 uveitis-clinical/animal model • 762 vitreoretinal surgery • 554 immunohistochemistry  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.