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Marisa Arcos, Alicia E Farinati, Maria T Diluca, Martin G Santalucia, Biofilms on Lenses; Pseudomonas aeruginosa Biofilm (BF) Adhesion and Dispersion on lenses under Ketorolac (KL) and Bromfenac (BR) influence.. Invest. Ophthalmol. Vis. Sci. 2014;55(13):6259.
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Pseudomonas aeruginosa (PA) is one of the most important bacteria in eye infections like keratitis, scarring and even blindness when build up BF on lenses (CL). The natural bacterial remotion is ineffective when the eye is covered by a CL. It is demonstrated that KL reduces the formation of Staphylococcus epidermidis BF that caused endophtalmitis. Objective: to investigate the effect of KL and BR in vitro on PA BF.
Material and methods: we use 20 PA clinical isolates. The BF was studied quantitatively on two supports: glass coupon (GC) and new CL. Each isolate(inoculum 103 cel/ml), was incubated at 35 C in Trypticase soy Broth with the GC and the CL (BF system -BFS). Adhesion and Dispersion: after 1 and 4 hours we added KL (0.18ug/mg)(group A) and BR (0.10ug/mg) (group B) to each BFS. The CL were observed with optical microscopy (400x) without any tinction. The GC were observed with optical and fluorescence microscopy, stained with crystal violet (CV) and acridine orange respectively. In a similar way we proceeded with 96-well microtiter for quantitative evaluation and were observed with a microplate reader (RT-2100 absorbance mode: 450 nm), stained with CV.
Results: Adhesion: we did `t find any evidence of KL or BR activity in any of the supports. Dispersion: In microtiter plate absorbance reader reduction in 70% of color was observed with KL and 67%with BR. It was correlated with the qualitative assay on both BFS in the two groups.
Conclusions: we noticed that KL and BR increased dispersion in all the supports. These results are important for considering their use in the CL associated Infections. The difference between KL and BR is very low.
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