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Ji-Eun Lee, Yan Sun, Eric Pearlman; Inhibition of Corneal Inflammation by the Lipid Mediator of Resolvin. Invest. Ophthalmol. Vis. Sci. 2014;55(13):6277.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the role of naturally occurring small molecule lipid mediator, resolvin in a murine model of corneal infiltrates.
C57BL/6 mouse corneas were abraded and treated with resolvin or placebo either before or after stimulation with antibiotic-killed Pseudomonas aeruginosa and Staphylococcus aureus. Corneal infiltrates were detected by in vivo confocal microscopy and by immunohistochemistry for neutrophils. The effect of resolvin on stimulated human corneal epithelial cells (HCECs), macrophages, and neutrophils was also assessed.
Resolvin significantly inhibited CXC chemokine production in the cornea and development of corneal infiltrates, specifically neutrophils, in response to stimulation with both P. aeruginosa and S. aureus. When the antagonist was applied after bacterial stimulation, neutrophil infiltration was also inhibited, although a higher concentration was needed. Furthermore, IL-8 production by both bacteria-stimulated HCECs, macrophages and neutrophils was also significantly reduced.
Resolvin is a highly effective antagonist of both gram positive (S. aureus) and gram negative (P. aeruginosa)-dependent corneal inflammation, which is suggested to be used as antiinflmmatory agent in bacterial keratitis.
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