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Jobu Sugita, Yosuke Asada, Hiroyuki Kawano, Nobuyuki Ebihara, Akira Murakami, Susumu Nakae, Akira Matsuda, Juntendo University Graduate School of Medicine; The role of interleukin-33 expression in retinal tissue. Invest. Ophthalmol. Vis. Sci. 2014;55(13):708.
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Interleukin-33 (IL-33) is a IL-1 family cytokine, known to have pro-fibrotic function as other Th2 cytokines. It is also proposed that IL-33 work as an alarmin in response to cellular injury. In this study, we investigated IL-33 expression in retinal tissue and its role for retinal fibrotic responses.
Immunohistochemical analysis and Western blotting analysis of mouse retinal tissue (BALB/C strain) were carried out with anti-mouse IL-33 antibody (R&D systems). Organ culture of mouse retinal tissue was carried out using OPTI-MEM medium (Invitrogen) in 96 well culture dishes, and IL-33 concentration in the supernatant was quantified by mouse IL-33 ELISA kit (e-bioscience). IL-33 expression after needle injury of the retina was evaluated with realtime PCR. The effect of recombinant IL-33 injection to the vitreous cavity was analyzed using IL-33 knockout mouse.
Immunohistological analysis showed that IL-33 expression was observed in the nucleus of Muller cells of retinal tissue. Western blotting analysis of retinal tissue confirmed IL-33 protein expression. IL-33 protein produced from organ culture of retinal tissue increased from 403pg/ml at 1 hour to 1761pg/ml at 24 hour. Statistically significant IL-33 mRNA upregulation was observed 24 hour after needle injury. Recombinant IL-33 injection to the vitreous induced TGF-beta1, TIMP-1 and COL1A1 expression in the retinal tissue.
Profibrotic cytokine IL-33 is expressed in mouse retinal tissue and may have some roles for fibrotic responses of the eye.
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