April 2014
Volume 55, Issue 13
ARVO Annual Meeting Abstract  |   April 2014
Wnt-signaling and the formation of Muller glia-derived progenitor cells in the chick retina
Author Affiliations & Notes
  • Donika Gallina
    Neuroscience, The Ohio State University, Columbus, OH
  • Lillia N Steffenson
    Neuroscience, The Ohio State University, Columbus, OH
  • Andrew J Fischer
    Neuroscience, The Ohio State University, Columbus, OH
  • Footnotes
    Commercial Relationships Donika Gallina, None; Lillia Steffenson, None; Andrew Fischer, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science April 2014, Vol.55, 828. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Donika Gallina, Lillia N Steffenson, Andrew J Fischer; Wnt-signaling and the formation of Muller glia-derived progenitor cells in the chick retina. Invest. Ophthalmol. Vis. Sci. 2014;55(13):828.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: One of the signaling pathways that may regulate the activity of retinal glia and Müller glia-derived progenitor cells (MGPCs) is the Wnt pathway. During ocular development, Wnt-signaling is known to have many important functions that involve patterning the anterior optic structures and suppressing retinal development. Studies in zebrafish have shown that activation of Wnt-signaling is required for MGPC-mediated retinal regeneration in vivo. Studies in mammalian retina have shown that Wnt-signaling stimulates the Müller glia to re-enter the cell cycle in retinal explants in vitro. We investigate the role of the Wnt pathway in the formation of MGPCs in the avian retina in vivo.

Methods: : Immunocytochemistry was used to study the expression pattern of nuclear β-catenin (a read-out of Wnt-signaling) in mature and damaged chick retinas. Wnt- signaling inhibitors (XAV939) or a combination of Wnt-signaling activators (GSK3β-inhibitors) were injected into the vitreous chamber of normal and damaged eyes. Retinas were processed for cell proliferation and glial reactivity. qRT-PCR was used to measure retinal levels of different Wnt-related and progenitor genes. Retinal damage was induced by an intraocular injection of N-Methyl-D-aspartate (NMDA).

Results: We found that nuclear β-catenin is present in many neurons, but not Müller glia in mature retina. In damaged retina nuclear β-catenin is expressed by MGPCs (need confirmation). Furthermore, NMDA-induced retinal damage leads to activation of Wnt-signaling, indicated by the regulation of transcripts of Wnt-target genes. Inhibition of Wnt-signaling suppressed the formation of proliferating MGPCs, and activation of Wnt-signaling expanded the population of the proliferating MGPCs.

Conclusions: We propose that Wnt-signaling is important for the proliferation of MGPCs in damaged avian retina in vivo. Our data suggest a role of Wnt-signaling in the regeneration of the chick retina.

Keywords: 603 Muller cells • 699 retinal glia • 687 regeneration  

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.