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Marcus Kernt, Anselm Kampik, Hartwig Becker; CYTOPROTECTIVE EFFECTS OF BLUE LIGHT FILTERING INTRAOCULAR LENS WITH LIGHT-ADAPTIVE TRANSMISSION ON HUMAN RETINAL PIGMENT EPITHELIUM BY REDUCING PHOTOTOXIC EFFECTS ON VEGF-A, BAX, AND BCL-2 EXPRESSION. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1064.
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© ARVO (1962-2015); The Authors (2016-present)
Recently, a novel concept of a blue light filtering IOL with light-adaptive transmission (TA-IOL) has been introduced, to prevent the retina from potential damage by blue light in pseudophacia. This study compares the possible protective effects of the TA-IOL to an untinted, UV-absorbing IOL, with regards to light-induced stress on human RPE.<br />
A novel IOL, characterized by a central UV and blue light filtering zone, a surrounding transition zone and a clear, UV only absorbing periphery, was compared to a standard UV-absorbing IOL (UVa-IOL). Primary human retinal pigment epithelium (RPE) cells were exposed to white light, and either a TA-IOL or an UVa-IOL was placed in the light beam. After 15 to 60 minutes of irradiation, the viability of the cells was determined by a colorimetric test (MTT) and a microscopic live dead assay. The expression of VEGF-A, BAX, and Bcl-2 and their mRNA was determined by RT-PCR and Western blotting.
Without any IOL, white-light exposure decreased cell viability compared to the non-irradiated control in a time-dependent manner. The UV- and blue-light filtering of the TA-IOL attenuated light-induced cell damage significantly more than the UVa-IOL. RT-PCR and Western blotting yielded a significant, time-dependent decrease of Bcl-2 and increase of BAX and VEGF-A. This decrease of Bcl-2 and increase of BAX and VEGF-A was significantly less with the TA-IOL than with the UVa-IOL.
Both TA-IOL and UV- and UVa-IOL reduce light-induced RPE-damage. The TA-IOL reduced damage more than the conventional IOL. The presented concept of a TA-IOL may help to protect the retina against light-induced damage and reduce potential negative influences of blue light filtering IOLs on the quality of vision under low light conditions.
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