June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
A pilot study of creating keratoconus model by collagenase type II
Author Affiliations & Notes
  • JING QIAO
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • XIAOMING YAN
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Wenjing Song
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Yun Tang
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Bei Rong
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Haili Li
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Songlin Yang
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Yaqi Yin
    Institute of mechanics, Chinese Academy of Sciences; the State Key Laboratory of Nonlinear Mechanics, Beijing, China
  • Yuan Wu
    Department of Ophthalmology, Peking University First Hospital; Key Laboratory of Vision Loss and Restoration, Ministry of Education, Beijing, China
  • Footnotes
    Commercial Relationships JING QIAO, None; XIAOMING YAN, None; Wenjing Song, None; Yun Tang, None; Bei Rong, None; Haili Li, None; Songlin Yang, None; Yaqi Yin, None; Yuan Wu, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 1113. doi:
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      JING QIAO, XIAOMING YAN, Wenjing Song, Yun Tang, Bei Rong, Haili Li, Songlin Yang, Yaqi Yin, Yuan Wu; A pilot study of creating keratoconus model by collagenase type II. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1113.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To set up an experimental animal model of keratoconus using collagenase type II and evaluate corneal curvature changes.

Methods: 20 mortem New Zealand white rabbit corneas were divided into 4 groups: group 1(15 mg/ml collagenase type II with 15% dextran, N=5), group 2(10 mg/ml collagenase type II with 15% dextran, N=5), group 3(5 mg/ml collagenase type II with 15% dextran, N=5) and group 4(the control group,15% dextran, N=5). After epithelial debridement, corneas were mounted and pressured on artificial anterior chambers,then different kinds of solution was applied to corneas for 1 hour. Corneal curvature was measured before and after collagenase exposure at different intraocular pressure levels(15mmHg, 30mmHg,45mmHg). The results were analyzed statistically.

Results: After exposure, changes of Kmean in group 1,group 2,group 3 and group 4 were 6.33±4.05D, 0.93±1.13D, 0.67±1.66D and -0.26±0.77D respectively at 15mmHg; 5.36±2.39D, 1.49±1.70D, 1.37±2.07D and -0.77±1.11D respectively at 30mmHg; 7.29±3.39D, 2.41±2.37D, 1.47±2.63D and -0.56±1.35D respectively at 45mmHg. Compared to group 4, a statistically significant increase in Kmean across all the intraocular pressure levels was seen in group 1 (p<0.05).However, group 2, group3 and group 4 were not significantly different from group 4(p>0.05).

Conclusions: 15 mg/ml collagenase type II could induce significant increase in corneal curvature, which might be considered as a method of building the rabbit model of keratoconus.

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