INNOVATIVE USE OF SPECTRALIS OCT AUTOFLUORESCENCE ON TRACKING EX-VIVO HUMAN CORNEAL ENDOTHELIAL CELL COLONIES.

Abstract

 

Purpose

 

DEVELOP A NEW METHOD TO TRACK ADHESION AND MIGRATION PATTERNS OF HUMAN CORNEAL ENDOTHELIAL CELLS AFTER EX-VIVO IMPLANTATION.

 

Methods

 

Human corneal endothelial cells previously cultured in vitro were divided in two groups: exposed or non-exposed to magnetic nanoparticles. Those cells were used as an attempt to regenerate our ex-vivo host corneas. Cell colonies were tracked after 7 days using an scanning laser ophthalmoscope with a excitation light of 488 nm. Human corneal endothelial cells previously cultured in vitro were divided in two groups: exposed or non-exposed to magnetic nanoparticles. Those cells were used as an attempt to regenerate our ex-vivo host corneas. Cell colonies were tracked after 7 days using an scanning laser ophthalmoscope with a excitation light of 488 nm.

 

Results

 

Human corneal endothelial cells showed no toxicity when exposed to the studied nanoparticle(p=0.507). Both, non-magnetic and magnetic cells were found attached to posterior surface of corneas after 7 days of ex-vivo cultures and visualized by C-SLO Spectralis autofluorescence mode.

 

Conclusions

 

Human corneal endothelial cells from magnetic and non-magnetic groups attached to corneal posterior surface on ex-vivo hosts.