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Mario Matthaei, Johannes Gillessen, Philipp S Muether, Robert Hoerster, Bjoern O Bachmann, Arno Hueber, Claus Cursiefen, Ludwig M Heindl; Long-term increase of TGF-β1, TGF-β2 and MCP-1 in the aqueous humor of pseudophakic Fuchs Dystrophy Eyes. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1170.
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To analyze the specific expression of distinct epithelial-mesenchymal transition (EMT)-associated proteins in the aqueous humor of phakic and pseudophakic FECD eyes.
Fifty aqueous humor samples from phakic FECD eyes (FECDph), from pseudophakic FECD eyes with more than one year post-cataract surgery (FECDpsph) and from cataract controls without FECD (Controlcat) were collected during Descemet membrane endothelial keratoplasty (DMEK) or cataract surgery. The expression of EMT-associated proteins (TGF-β1, TGF-β2, TGF-β3, MCP-1, FGF-basic, TNF-α, Il-1ß) was determined by multiplex bead assay and comparatively analyzed.
FECDpsph eyes showed significantly elevated expression compared to FECDph and Controlcat groups respectively for TGF-β1 (p=0.0011 and p<0.0001), TGF-β2 (p=0.0288 and p=0.0076) and MCP-1 (p=0.0007 and p<0.0001). No differences in EMT-associated protein levels were detected comparing FECDph eyes and Controlcat eyes.
Cataract surgery causes long-term elevated levels of distinct EMT-associated proteins (TGF-β1, TGF-β2, MCP-1) in the aqueous humor of FECD eyes. This may contribute to accelerated FECD progression post cataract surgery. No alterations in EMT-associated protein levels were found in phakic FECD eyes.
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