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Zaid Mammo, Chandra Bala, Jing Xu, Paula yu, Yifan Jian, David Albiani, Andrew Kirker, Andrew Merkur, Dao-Yi Yu, Marinko V Sarunic; Evaluating the Utility of Speckle-Variance Optical Coherence Tomography for Assessing the Human Macula. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1647.
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© ARVO (1962-2015); The Authors (2016-present)
1) To demonstrate the utility of speckle variance optical coherence tomography (svOCT) for imaging human macular capillary networks. 2) To compare svOCT images of the macula with fluorescein angiography (FA) and histological images in a qualitative and quantitative manner.
A total of twelve eyes from six subjects had both svOCT and FA imaging. Manual tracing of macular vasculature images from the two modalities were performed and quantitative comparisons of capillary density was determined. Morphometric dimensions of the foveal avascular zone (FAZ) were quantified in 26 healthy human eyes after svOCT imaging. These results were compared to human histology data acquired from 11 cadaveric specimens.
Mean age of subjects in the svOCT group and histology groups were 28.1 ± 4.6 and 45.2 ± 17.8 years (P < 0.001), respectively. Similar to histology, svOCT images were able to distinguish the inner and outer macular capillary networks (Figure 1). The svOCT images had an average capillary density of 31.2 ± 5.56 % versus19.3 ± 6.50 % for the FA group (p= < 0.001) (Figure 2). The reproducibility of this technique was high with no difference in density measurements between repeated tracings (P = 0.195). FAZ perimeter measurements were significantly greater in svOCT images (2.1 ± 0.4 vs. 1.6 ± 0.7 mm, P < 0.010) as were FAZ area measurements (0.3 ± 0.1 vs. 0.1 ± 0.1 mm2, P < 0.010).
svOCT appears to be a useful technique for delineating the morphometric characteristics of capillary networks in the human macula. Advantages of this modality include its non-invasive nature and its capacity to isolate the inner and outer macular networks. It therefore has the potential to supplement FA in the assessment of the retinal vasculature. Further work is required to validate the utility of this device for managing patients with retinal microvascular diseases.
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