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Ji-Eun Lee, Haksun Yu; Cytopathic Mechanisms of Acanthamoeba. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):1887.
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© ARVO (1962-2015); The Authors (2016-present)
To report the mechanism of cytolysis of corneal epithelial cells by Acanthamoeba using streroid.
After quantifying the corneal epithelial cell cytolysis capacity of steroid-treated Acanthamoeba by a spectrophotometric assay, collagenase activity of Acanthamoeba was measured. In addition, flow cytometric analyses with annexin V were used to identify the nature of the corneal epithelial cell response to Acanthamoeba. Corneal epithelial cells were preincubated with various concentrations of caspase inhibitors and apoptotic response was assayed by LDH assay.
Steroid-treated Acanthamoeba induced a significant cytopathic effect on corneal epithelial cells compared with untreated organisms. The enhanced cytolysis of epithelial cells by steroid-treated Acanthamoeba was due to increase elaboration of collagenase. Apoptotic changes in corneal epithelial cells were detected by flow cytometry and specific inhibitor of caspase-3 significantly reduced the LDH activity induced by Acanthamoeba.
These findings indicated that exposure of Acanthamoeba to steroid increased the pathogenicity of the organisms, and Acathamoeba induced apoptosis in corneal epithelial cells associated with caspase-3 pathway.
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