Purchase this article with an account.
J Cameron Millar, Tien N Phan, Iok-Hou Pang, Abbot F Clark; STRAIN & AGE SURVEY OF MOUSE AQUEOUS HUMOR DYNAMICS.. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2002.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Mouse aqueous humor dynamics (AHD) have been studied by several laboratories, but there is no clear consensus. We hypothesize that strain/age differences may play a role. This study evaluated differences in AHD among younger and older age groups of several strains.
A/J, BALB/cJ, C3H/HeJ, & C57-BL/6J mice (all ♀) were studied. Animals in each strain were divided into 2 cohorts (young, 2½-4½ mo; aged, 10-12 mo) (N=5-7/cohort). Baseline (BL) intraocular pressure (IOP) was measured (TonoLab). AHD parameters (outflow facility (C), episcleral venous pressure (Pe), aqueous humor formation (Fin), uveoscleral outflow (Fu)) were established (constant flow infusion).
BL IOP did not exhibit age-dependence, except in C57-BL/6J animals, which showed an age-related increase (13.90±0.25 vs 15.43±0.21 mmHg, P=0.00048). In other strains, BL IOP (young vs aged) was: 12.42±0.08 mmHg vs 12.56±0.10 mmHg (A/J), 11.35±0.11 mmHg vs 11.68±0.20 mmHg (BALB/cJ), 13.27±0.17 mmHg vs 13.65±0.09 mmHg (C3H/HeJ). Fu decreased with age in the BALB/cJ strain (0.066±0.022 μL/min vs. 0.011±0.007 μL/min, P=0.0055, ↓83%), the C3H/HeJ strain (0.109±0.028 μL/min vs 0.024±0.009 μL/min, P=0.015, ↓78%), the C57-BL/6J strain (0.080±0.022 μL/min vs 0.012±0.008 μL/min, P=0.013, ↓85%), and the A/J strain (0.12±0.048 μL/min vs. 0.036±0.031 μL/min, NS, ↓70%). Fin decreased with age in the A/J strain (0.202±0.035 vs 0.116±0.029 μL/min, P=0.047) and the C3H/HeJ strain (0.140±0.027 vs 0.065±0.006, P=0.021). Fu as % of Fin decreased with age in the BALB/cJ strain (37.41±10.24% vs 7.91±4.93%, P=0.038), the C3H/HeJ strain (71.17±8.59% vs 34.44±8.59%, P=0.029), the C57-BL/6J strain (52.66±13.66% vs 14.23±8.24%, P=0.033) and the A/J strain (53.93±14.51% vs 24.77±11.91%, NS). C in the BALB/cJ strain increased with age (0.039±0.005 vs 0.024±0.002 μL/min/mmHg, P=0.02). In C3H/HeJ and C57-BL/6J strains C trended towards an increase with age. Pe was (young vs aged): 6.56±0.12 mmHg vs 6.49±0.08 mmHg (A/J), 5.96±0.11 mmHg vs 5.99±0.03 mmHg (BALB/cJ), 8.52±0.09 mmHg vs 8.60±0.06 mmHg (C3H/HeJ), 8.73±0.16 mmHg vs. 8.88±0.08 mmHg (C57-BL/6J). Pe was not age dependent but was higher in pigmented animals.
In mice, with age, Fu diminishes (70-85%), and Fin tends to diminish, as in humans and NHPs. C tends to increase with age as animals progress to early middle-life. There are also strain differences in Fu, IOP, and Pe in young and aged animals, and C and Fin in aged animals.
This PDF is available to Subscribers Only