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Shoumyo Majumdar, Qiongyu Guo, Anirudha Singh, Oliver D Schein, Morgana Trexler, Jennifer Elisseeff; Fabrication of cornea-mimetic, cyclodextrin-collagen based biomaterials for corneal transplantation. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2044.
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© ARVO (1962-2015); The Authors (2016-present)
We have developed and characterized collagen I based biomimetic ‘vitrigel’ biomaterials that exhibit properties of high transparency, suturability and collagen fibril alignment for application as a regenerative membrane for corneal repair. We utilized the effect of interactions between collagen and cyclodextrins (CD), a family of cyclic oligosachharides, in order to maintain transparency at high thicknesses. Following this, we have also tested a number of functionalized cyclodextrins, to assess the modulation of structural properties in the vitrigel membranes.
Vitrigels were prepared as previously described (Biomaterials 33 (2012) 8286-95) with minor modifications to incorporate cyclodextrins (alpha-, beta-, and gamma- cyclodextrins). In addition, vitrigels with modified CDs with different functional groups including methyl, thiol, carboxylic acid, phosphate, were also evaluated. Light transmission of the membranes was measured in a Synergy 2 microplate reader (Biotek). Vitrigel samples were processed for TEM and stained with uranyl acetate and images were taken with a Philips CM120 TEM.
CD-incorporated collagen (CD-Col) vitrigel membranes exhibited high degrees of transparency. Light transmission (Figure 1) varied between types of CD incorporated; these values were significantly higher than vitrigels without CD. In TEM images (Figure 2B), some functionalized CD-Col vitrigels demonstrate unique self-assembly properties, wherein a lamellar ultrastructure with aligned collagen, similar to native cornea, is obtained. This effect is also be observed in macro-scale, as the lamellae can be carefully separated.
Functionalized cyclodextrins demonstrate the ability to organize collagen fibrils, allowing higher order self-assembly. This phenomenon further justifies the increased transparency seen in CD-Col membranes. In addition, this study highlights the influence of small molecule interactions with collagen during fibril formation, and illustrates the importance of functionalized CD-Col membranes as potential corneal transplant materials.
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