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Ankita Kotnala, Senthilkumari S, Nabanita Halder, B Jayaram, Atul Kumar, Thirumurthy Velpandian; Identification and evaluation of the levels of various age related lipofuscin components in human macula . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2380.
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© ARVO (1962-2015); The Authors (2016-present)
Accumulation of lipofuscin in human retinal pigment epithelium is reported to be one of the causative factors in the pathogenesis of Age related Macular Degeneration.Therefore, the present study was undertaken to identify and evaluate the levels of various lipofuscin components in normal age matched aging Indian donor eyes.
Donor eyes (Group 4 ,above 80 yrs; N=36, Group 3, 60-80 yrs; N=60, Group 2, 40-60 yrs ;N=13,Group 1, below 40 yrs ; N=44) obtained after the removal of cornea for transplantation were collected from the Eye Bank of Arvind Hospital, Marudai with prior approval from the Human Ethics Committee. 8mm macular portion was punched, extracted and sent to AIIMS for analysis. Lipofuscin components (A2-glycerophosphoetahnolamine(A2GPE) All trans retinal dimer (ATRD), A2-dihydrophosphoethanolamine (A2DHPE)), of age matched samples were analyzed with a newly developed LC- ESI/MS/MS method.
Several lipofusion components viz A2GPE, ATR-dimer, ATRDHPE, A2E, isomers of A2E, monofuran A2E, mono-peroxy A2E etc were found. Presence of A2GPE , ATRD and A2DHPE were further confirmed by their fragmentation pattern. In the group 4,3 & 2, A2GPE levels were found to increase 41, 39, 7 % more than the levels found in macula belong to >40 years group (group 1). Increasing A2GPE levels between Group 4 and 1 were found to be statistically significant. In age group 4,3 & 2 levels were found out to be 11, 8 ,8 % more then that of levels below >40 years group(group 1). Increasing A2DHPE levels were found to be significant for group 1. Similar increasing trend found for ATRD. In the group 4, 3 & 2 , ATRD levels were found out to be 49, 73 and 24 % more then the levels below>40 years age group (group 1). Increasing ATRD levels were found to be significant for group 1, 2 and 3.
This study further confirmed the presence of several lipofusion components such as A2GPE, ATR-dimer, ATRDHPE, A2E, isomers of A2E, monofuran A2E, mono-peroxy A2E etc. in the human macular extracts. Age related increase in A2E has already been reported in the literature, however, for the first time the present study shows that along with A2E increasing levels of A2GPE , ATRD and A2DHPE can also contribute towards the pathogenesis of AMD. Further studies are in progress to elucidate the levels of oxidized products of lipofusion components.
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