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Yoko Okunuki, Eiichi Hasegawa, Ryoji Yanai, Kaylee Smith, Alexandra Castillejos, Kip M Connor; The effect of dietary supplementation with omega-3 and omega-6 long-chain polyunsaturated fatty acids on retinal detachment. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):25.
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© ARVO (1962-2015); The Authors (2016-present)
Retinal detachment (RD) is a sight threatening disorder characterized by the physical separation of the photoreceptors from the retinal pigment epithelium. As a result, the detached photoreceptors undergo apoptosis and programmed cell death. It has been suggested that omega-3 long-chain polyunsaturated fatty acid (LCPUFA) and its derivatives have the neuroprotective and anti-inflammatory properties. On the other hand, omega-6 LCPUFA has been suggested as pro-inflammatory. In this study, we examined the effect of dietary supplementation, with omega-3 and omega-6 LCPUFAs, on photoreceptor cell death after RD.
C57BL/6J mice were fed a diet enriched with either omega-3 or omega-6 LCPUFAs for 2 weeks. Then RD was induced by subretinal injection of 4 ul of sodium hyaluronate into C57BL/6J mice. RD-induced photoreceptor cell death was assessed by TUNEL staining at 24, 48, 72hrs, and 7 days after the surgery. Additionally, outer nuclear layer thickness was evaluated 7days after the surgery.
The number of TUNEL positive cells in the outer nuclear layer was not significant in mice fed either omega-3 or omega-6 LCPUFAs 24hrs post retinal detachment (p=0.1). However at 48hrs post detachment omega-3 fed mice had a significant, albeit small, decrease in the number of TUNEL positive cells (p=0.04) compared to their omega-6 fed counterparts. There was no difference in the number of TUNEL positive cells at 72hrs and 7days after RD. Outer nuclear layer thickness at 7 days after RD was not different between omega-3 and omega-6 LCPUFAs fed mice.
We demonstrated that dietary supplementation with omega-3 and omega-6 LCPUFAs does not likely play a significant effect on photoreceptor cell death after RD. This suggests that omega-3 LCPUFA lack a neuroprotective effect on photoreceptor cell death after RD.
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