June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Measuring Fluid Outflow in Rabbits Post Glaucoma Implant Surgery with iMvalv
Author Affiliations & Notes
  • Fabio Ariel Guarnieri
    Bioengineering, CIMEC, Santa Fe, Argentina
    Lab BioMEMS, UNER, Oro Verde, Argentina
  • Rodrigo M Torres
    Instituto Lódolo, Paraná, Argentina
  • Footnotes
    Commercial Relationships Fabio Guarnieri, CONICET (F), CONICET (P), iMvalv (I), UNER (F); Rodrigo Torres, iMvalv (C)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 2696. doi:
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    • Get Citation

      Fabio Ariel Guarnieri, Rodrigo M Torres; Measuring Fluid Outflow in Rabbits Post Glaucoma Implant Surgery with iMvalv. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2696.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

The iMvalv is an ocular implant with MEMS technology to produce aqueous humor drainage with an active mechanism. It was built with silicone multiple layers, for their plate and tube and has the capability of an active mechanism powered by a spiral antenna and a hybrid microcircuit. A first exploratory in-vivo implantation study in rabbits was performed and hydraulic conductivity was assessed in vivo and compared with in vitro testing.

 
Methods
 

Four New Zeland male rabbits underwent insertion of a iMvalv implant into the anterior chamber of their right eyes. Left eyes were used as controls. Both eyes had outflow measurements performed at day 44 after surgery.<br /> Measurements were performed by cannulating the drainage tube ostium in situ with a needle attached to a fluid column at 10, 15, 20 and 25 mmHg. The drop in the fluid column was measured by a video camera. For the control eyes the anterior chamber of the unoperated fellow eye was cannulated with a needle attached to a fluid column. The drop in the fluid column was also measured. Then, Perkins tonometry was performed in order to calibrate previous measurements (pre and post surgery) with this fluid column pressure.

 
Results
 

Perkins tonometry and manometric readings were compared in control eyes. Manometric readings of 10, 15, 20 and 25 mmHg gave mean values of 5, 6, 9 and 9.5 mmHg from simultaneous Perkins tonometries. In one implanted eye, a manometric column 20 mmHg was dropped to 11.4 mmHg and from 15 to 14.4 mmHg. In a second implanted eye the drop was from 21.6 to 21.1 mmHg. After euthanasia (day 50) conductivity of each valve was verified with a manometric column and a syringe pump concluding that one implant had still high conductivity while the other had low conductivity.

 
Conclusions
 

We described a model to directly measure hydraulic conductivity in a rabbit glaucoma surgery implant with iMvalv and calibration of Perkins tonometry. The drainage of each implant can be reliably quantified and measured.  

 
Perkins Tonometry and manometric readings in control eyes.
 
Perkins Tonometry and manometric readings in control eyes.
 
 
Imvalv tube cannulated by a needle with a manometric fluid column for conductivity measurements.
 
Imvalv tube cannulated by a needle with a manometric fluid column for conductivity measurements.

 
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