June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Total Retinal Blood Flow Measurements with En Face Doppler Optical Coherence Tomography in Primary Open-Angle Glaucoma
Author Affiliations & Notes
  • ByungKun Lee
    Elec Eng & Comp Sci and Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • Chandrasekharan Krishnan
    New England Eye Center, Tufts Medical Center, Boston, MA
  • Woo Jhon Choi
    Elec Eng & Comp Sci and Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • Mehreen Adhi
    New England Eye Center, Tufts Medical Center, Boston, MA
  • Talisa de Carlo
    New England Eye Center, Tufts Medical Center, Boston, MA
  • Adam T Chin
    New England Eye Center, Tufts Medical Center, Boston, MA
  • Chen D Lu
    Elec Eng & Comp Sci and Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • Jay S Duker
    New England Eye Center, Tufts Medical Center, Boston, MA
  • Joel S Schuman
    Ophthalmology, University of Pittsburgh School of Medicine, Pittsburgh, PA
  • James G Fujimoto
    Elec Eng & Comp Sci and Research Lab of Electronics, Massachusetts Institute of Technology, Cambridge, MA
  • Footnotes
    Commercial Relationships ByungKun Lee, None; Chandrasekharan Krishnan, None; Woo Jhon Choi, None; Mehreen Adhi, None; Talisa de Carlo, None; Adam Chin, None; Chen Lu, None; Jay Duker, Carl Zeiss Meditec, Inc. (F), Optovue, Inc. (F); Joel Schuman, Carl Zeiss Meditec, Inc. (P); James Fujimoto, Carl Zeiss Meditec, Inc. (P), Optovue, Inc. (I), Optovue, Inc. (P)
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 2747. doi:https://doi.org/
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      ByungKun Lee, Chandrasekharan Krishnan, Woo Jhon Choi, Mehreen Adhi, Talisa de Carlo, Adam T Chin, Chen D Lu, Jay S Duker, Joel S Schuman, James G Fujimoto; Total Retinal Blood Flow Measurements with En Face Doppler Optical Coherence Tomography in Primary Open-Angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):2747. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose
 

Total retinal blood flow (TRBF) measurement with en face Doppler optical coherence tomography (OCT) does not require vessel angle information and therefore enables fully automatic calculation of blood flow with improved measurement repeatability. High-speed SS-OCT imaging can resolve blood flow pulsatility, allowing accurate mean TRBF measurement. We investigate TRBF using en face Doppler OCT in patients with primary open-angle glaucoma (POAG).

 
Methods
 

TRBF was measured in one randomly selected eye for each of 13 patients with POAG (age 69.4±3.9yo) and 6 normal subjects (age 62.5±10.7yo) using a high-speed SS-OCT prototype operating at 400kHz axial scan rate and 1050nm wavelength. Volumetric data comprising 600×80 axial scans over a 1.5mm×2mm area at the optic disc was acquired at 7.1 volumes per second, fast enough to resolve flow pulsatility in the central retinal artery. The volume scan was repeated 24 times in 3.4 seconds of total acquisition time. Automatic software calculation of TRBF was implemented. Mean TRBF was calculated as the average of TRBF over the cardiac cycle.

 
Results
 

Mean TRBF was 35.9±7.4μL/min in eyes with POAG and 42.0±8.7μL/min in normal eyes. The difference between the two groups was not statistically significant (P>0.05, one-tailed Welch's t-test). This differs from several previous reports where eyes with POAG exhibited significantly less TRBF than normal eyes.

 
Conclusions
 

En face Doppler OCT measurement of TRBF in patients with POAG and normal subjects was performed using a high-speed SS-OCT instrument. A statistically significant difference in TRBF was not observed between POAG and normals. TRBF may not be a sensitive diagnostic marker for detecting glaucoma because of the large variation in the normal population, although it may be useful for assessing progression. Functional flow response to stimulus may be a more sensitive marker which warrants further investigation.  

 
Fig. 1. Automatic vessel segmentation and flow calculation in an eye with POAG. (A) Fundus intensity projection. (B, C) Pulsatile fluctuation of arterial flow velocity in a selected en face plane. (D, E) Intensity and Doppler phase images of the extracted B-scan at the marked position in (A, B). Scale bars 250µm.
 
Fig. 1. Automatic vessel segmentation and flow calculation in an eye with POAG. (A) Fundus intensity projection. (B, C) Pulsatile fluctuation of arterial flow velocity in a selected en face plane. (D, E) Intensity and Doppler phase images of the extracted B-scan at the marked position in (A, B). Scale bars 250µm.
 
 
Fig. 2. Automatic calculation of mean TRBF. Blue line represents the pulsatile TRBF waveform and red markers are the data points used for calculating mean flow.
 
Fig. 2. Automatic calculation of mean TRBF. Blue line represents the pulsatile TRBF waveform and red markers are the data points used for calculating mean flow.

 
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