Purpose
To investigate the effect of linezolid and vancomycine on biofilm formation on two different types of hydrophobic intraocular lenses.
Methods
Ica A, icaD and bap positive Staphylococcus epidermidis was used in this study for biofilm production. Biofilms were cultivated on disks of two different types of acrylic hydrophobic lenses( 25 % water content as Lens A, 2 % water content as Lens B) with different water contents.In the first protocol, the lenses were incubated for 24 hours after bacterial contamination. Then, 10 μl vancomycin from 1mg/0.1 ml or 15 μl linezolid from 2mg/ml solution was added to media containing the lenses. In the second protocol, the lenses were contaminated with bacteria and 10 μl vancomycin from 1mg/0.1 ml or 15 μl linezolid from 2mg/ml solution were added at the same time. In both protocols, after 24 hours incubation of the plates, the lenses were evaluated by spectrophotometry ( OD 620 nm) and number of bacteria was counted. The lenses were examined with scanning electron microscopy.
Results
In the first protocol, the number of bacteria was 5.1(Log10CFU/mL) for lens A and 5.3 for lens B with linezolid and 5.2 for lens A and 5.2 for lens B with vancomycin. In the second protocol, the number of bacteria was 1.4(Log10CFU/mL) for lens A and 1.4 for lens B with linezolid and 1.4 for lens A and 1.2 for lens B with vancomycin.
Conclusions
The effect of the linezolid and the vancomycine on biofilm formation on acrylic hydrophobic intraocular lenses was found similar with different water contents. Both were less effective when added on preformed biofilm.