Purchase this article with an account.
Fátima Sofía Magaña, Joaquín Arturo Quiroz, Gibrán Estua-Acosta, Mariana García, Yonathan Garfias; Characterization of endotoxin-induced uveitis in two different mouse strains. . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3100.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Uveitis is a systemic disease in which the uvea is inflamed. It has been reported that uveitis animal models can be generated in the laboratory. In this context, the aim of the present work was to determine the differences on clinical signs of uveitis as well as the eye leukocyte infiltration in two different mouse strains intraperitoneally exposed to LPS from E. coli.
Following the ARVO statement of animal care on investigation for visual sciences, male BALB/c and C57BL/6 mice from 6-8 weeks old were intreperitoneally injected with LPS from E. coli. Animals were clinically evaluated using stereoscopic microscopy. After 24 or 48 h of treatment, the mice were euthanized and the eyes were removed in order to determine histologic leukocyte infiltration. Student t test was performed to determine differences between groups taking a p<0.05 as statistically significative.
Intraperitoneally LPS induced clinical signs of uveitis in both mouse strains, which consisted in aqueous flare in the anterior chamber, miosis, iriitis and retinitis. In both strains, retinal blood vessels were igurgitated. These clinical signs appeared as soon as 24 h post LPS-injection and remain until 48 h. However, the clinical severity was higher in BALB/c mice in comparison to C57BL/6 mice. As expected, histological leucocyte infiltration was significantly higher (p<0.05) in BALB/c mice compared to C57BL/6 mice at 24 and 48 h post LPS-injection. Interesingly, there was a diferentially infiltration pattern, presenting a higher number of leucocytes infiltration in the posterior segment than in the anterior segment of the eye.
Taken together these results, suggest that endotoxin induced uveitis animal model depends not only on the administation via but also on the genetic background of the experimental mouse strain.<br /> <br /> Financial Support: This project was supported by CONACYT: SALUD 160286; CIENCIA BASICA 167438; DGAPA-PAPIIT IA203514; CVU: 406452.
This PDF is available to Subscribers Only