June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Macrophage Phenotype in the Ocular Surface of Experimental Murine Dry Eye Disease
Author Affiliations & Notes
  • In-Cheon You
    Ophthalmology, Chonbuk Ntl Univ & Hosp, Jeonju, Jeonbuk, Korea (the Republic of)
  • Eui-yong Kweon
    Ophthalmology, Chonbuk Ntl Univ & Hosp, Jeonju, Jeonbuk, Korea (the Republic of)
  • Terry G Coursey
    Baylor college of medicine, Houston, TX
  • Fang Bian
    Baylor college of medicine, Houston, TX
  • Flavia L Barbosa
    Baylor college of medicine, Houston, TX
  • Cintia S De Paiva
    Baylor college of medicine, Houston, TX
  • Stephen C Pflugfelder
    Baylor college of medicine, Houston, TX
  • Footnotes
    Commercial Relationships In-Cheon You, None; Eui-yong Kweon, None; Terry Coursey, None; Fang Bian, None; Flavia Barbosa, None; Cintia De Paiva, None; Stephen Pflugfelder, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 318. doi:https://doi.org/
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    • Get Citation

      In-Cheon You, Eui-yong Kweon, Terry G Coursey, Fang Bian, Flavia L Barbosa, Cintia S De Paiva, Stephen C Pflugfelder; Macrophage Phenotype in the Ocular Surface of Experimental Murine Dry Eye Disease. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):318. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To evaluate the phenotype of macrophages in the cornea and conjunctiva of C57BL/6 mice with induced experimental dry eye.

Methods: C57BL/6 mice exposed to desiccating stress (DS) were evaluated at 1, 5, and 10 days and C57BL/6 mice maintained non-stressed (NS) environment were used as controls. Whole eyes and adnexa were excised for histology or used for gene expression analysis. Location and phenotype of macrophages infiltrating the cornea and conjunctiva was evaluated by immunofluorescence analysis. Quantitative polymerase chain reaction evaluated macrophage markers and T-cell-related and inflammatory cytokine expression in cornea and conjunctiva.

Results: Immunofluorescence staining demonstrated that macrophages reside in the conjunctiva of control and dry eye mice and their number did not change with DS. Real-time RT-PCR demonstrated that the level of M1 macrophage marker, iNOS, increased prominently in the conjunctiva at DS10 days. In contrast, there was a non-significant decrease of the M2 marker Arg1 with DS. The levels of inflammatory cytokine, IL-12a mRNA transcript in the conjunctiva increased significantly at DS1 and decreased at DS5, while levels of IL-18 were significantly increased at DS10.

Conclusions: Macrophages reside in the ocular surface tissues of C57BL/6 mice. Although the number of macrophages in the conjunctiva does not change, evidence of inflammatory M1 activation after desiccating stress was observed. Better understanding of phagocyte diversity and activation in dry eye disease provide a basis for the development of phagocyte-targeted therapeutic strategies.

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