June 2015
Volume 56, Issue 7
Free
ARVO Annual Meeting Abstract  |   June 2015
Direct interaction of cochlin with mechanosensing channel TREK-1 in intraocular pressure regulation
Author Affiliations & Notes
  • Teresia Carreon
    Ophthalmology, University of Miami Bascom Palmer Eye Institute, Miami, FL
  • Carmen Piqueras
    Ophthalmology, University of Miami Bascom Palmer Eye Institute, Miami, FL
  • Aida Castellanos
    2. Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
  • Xavier Gasull
    2. Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain
  • Sanjoy K Bhattacharya
    Ophthalmology, University of Miami Bascom Palmer Eye Institute, Miami, FL
  • Footnotes
    Commercial Relationships Teresia Carreon, None; Carmen Piqueras, None; Aida Castellanos, None; Xavier Gasull, None; Sanjoy Bhattacharya, None
  • Footnotes
    Support None
Investigative Ophthalmology & Visual Science June 2015, Vol.56, 3254. doi:
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      Teresia Carreon, Carmen Piqueras, Aida Castellanos, Xavier Gasull, Sanjoy K Bhattacharya; Direct interaction of cochlin with mechanosensing channel TREK-1 in intraocular pressure regulation . Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3254.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: To determine the direct interaction between the extracellular matrix protein cochlin and the mechanotransducing channel TREK-1 in intraocular pressure regulation

Methods: TREK-1 mRNA was silenced in the DBA/2J mouse model in an effort to determine its effect on intraocular pressure. A sodium fluorescein dye assay along with a gel expansion assay was implemented to mimic the effect of the cochlin/TREK-1 interaction on trabecular meshwork cell conformation. The interaction was further characterized by a co-immunoprecipitation and the utilization of a yeast - two hybrid system. Patch - clamp whole cell recording was also performed in the presence or absence of cochlin in order to measure TREK-1 channel activity.

Results: TREK-1 mRNA silencing prevented a cochlin-induced increase in intraocular pressure (IOP). The presence of cochlin and TREK-1 together causes a spatial change in the cells of the trabecular meshwork as demonstrated by the increase of the fluorescein dye and the increase in collagen length in the gel expansion assay. A co- immunoprecipitation further demonstrated the increase of cochlin and TREK-1 specifically under shear stress and ionic stress conditions. The yeast-two hybrid system using a T7 promoter sequence demonstrated direct interaction between the bait (hCochlin) and TREK-1. In addition, cochlin interaction reduced TREK-1 channel currents as demonstrated by whole cell patch clamp recordings.

Conclusions: The results support the potential interaction occurring between cochlin and TREK-1 under stressful conditions, which in turn may result in extracellular matrix remodeling and an increase in IOP.

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