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BERTIN STEPHANE, Stephanie Zwillinger, Michel Paques, Christophe Baudouin, Antoine Labbe, Vincent M Borderie, Kate Grieve; Human trabeculum imaging with full-field optical coherence tomography. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3303.
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© ARVO (1962-2015); The Authors (2016-present)
There is currently no efficient method for the study of the human trabeculum without fixation histology. The aim of our study is to evaluate the performance of a full-field optical coherence tomography (FFOCT) compare to classical histology of human trabeculum and have a visualisation of its 3D structure.
Our study was carried out using a full-field OCT system from LLTech ®, developed for non-invasive imaging of tissue structures in depth. Images were acquired on human cornea donor and surgical specimens of trabeculectomy (GPAO). Classical histology was done on all surgical specimens.
The Full-field OCT device from LLTech® enables 2D visualisation in all plans and with ultrahigh resolution (1 micron in all directions). In human donor structure and cell visualisation was similar to classical histology. 3D visualisation of the trabeculum meshwork structure was determined without the histological fixation. In trabecuctomy or sclerectomy specimen of glaucoma eyes, we obtained with the same resolution and evaluated the trabeculum thickness, density and its containing volume.
Full-field OCT system provides trabeculum picture resolution equivalent to conventional histology but without fixation. High resolution slide in all directions and 3D visualisation of the human trabeculum and its modification in glaucoma could be easily studied with this technique as shown in our first results.
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