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Faryan Tayyari, Lee-Anne Khuu, Jeremy M Sivak, Shaun Singer, Michael H Brent, John G Flanagan, Christopher Hudson, Retina Research Group; Retinal Blood Oxygen Saturation and Aqueous Humor Biomarkers in Early Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3313.
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© ARVO (1962-2015); The Authors (2016-present)
To investigate the relationship between retinal blood oxygen saturation (SO2) and aqueous humor (AH) concentrations of protein biomarkers in diabetic patients with non-proliferative diabetic retinopathy (NPDR) and to compare them with those of control subjects.
The sample comprised 14 participants with mild-to-moderate NPDR (66.3 ± 9.1 years) and 17 age-matched controls (69.1 ± 5.7 years); all participants were previously scheduled for routine cataract extraction with intraocular lens implantation. At the start of surgery, AH was collected using a Sautter hydrodissection cannula (27G, 0.4x22mm; Geuder, Heidelberg, Germany). Multiplex cytokine analyses of 26 biomarkers, including Angiopoietin 2 (Ang 2), Epidermal Growth Factor (EGF), Hepatocyte Growth Factor (HGF) and Interleukin-8 (IL-8) were performed by BioPlex 200 system (Bio-Rad Laboratories, Inc., Hercules, CA, USA). Four weeks after uncomplicated surgery and 2 weeks after cessation of any anti-inflammatory eyedrops, non-invasive hyperspectral retinal (HR) imaging (prototype H-8.5 HR Camera, Optina, QC, Canada) was undertaken. Six repeated retinal images were acquired centered on the optic nerve head at wavelengths of 586 and 605nm using the HR camera for each subject.
Mean venular retinal blood oxygen saturation (p<0.001) and AH levels of HGF (p=0.018), Ang 2 (p=0.005), EGF (p=0.030) and IL-8 (p=0.034) were significantly higher in NDPR when compared to controls. This study demonstrated a correlation between venular retinal blood oxygen saturation and pro-angiogenic factors HGF (r=0.584, p=0.030), Ang 2 (r=0.592, p=0.026), and EGF (r=0.523, p=0.050), but did not find any correlation for IL-8 (r=0.435, p=0.120) even though this biomarker was significantly higher in the diabetic group.
The results of this study revealed a direct relationship between retinal blood SO2 and Ang 2, EGF and HGF but not IL-8.
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