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wenzhong Liu, Ji Yi, Ronil S. Shah, Brian Soetikno, Amani A Fawzi, Hao F Zhang; Longitudinal monitoring of choroidal neovascularization by OCT angiography in mice. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):3328.
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© ARVO (1962-2015); The Authors (2016-present)
Early detection of choroidal neovascularization (CNV) remains challenging in clinical practice by existing optical coherence tomography (OCT) and fluorescein angiography. Recently developed OCT angiography enhanced the blood flow contrast and suppressed the other static tissue signals, which allows us to visualize CNV directly. We hypothesize that OCT angiography can effectively detect CNV.
Adult pigmented mice were anesthetized by ketamine/xylene cocktail and subjected to laser treatment following established protocols of laser induced CNV around optic disc. Two OCT systems working in near infrared (NIR-OCT) and visible light range were used to provide complementary images for CNV characterization. A peripapillary area of 2x2mm2 centering optic nerve disk was imaged with both OCT systems. The OCT angiography scanning protocol scanned the same B-scan location twice and took the difference to enhance the blood flow contrast. The choroidal vasculature was extracted from the three-dimensional OCT angiography. OCT structural image and angiography were registered and compared between NIR and visible light OCT images. Four different CNV lesions were monitored longitudinally for 4 weeks after laser.
NIR-OCT provides better penetration depth into choroidal layer (sample image can be found in Figure 1) and the visible light OCT provides superb axial resolution down to 1 micrometer. We quantified the CNV area/volume from 3D angiography. We observed the choroidal vascular remodeling on both NIR and visible light OCT, which started around 4 days and peeked one week after laser induction. The CNV lesion gradually regressed afterwards.
Compared to conventional OCT images, OCT angiography provides enhanced contrast from vasculature and can be used to detect and monitor the progression of CNV.
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