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Merle Fernandes, Brinda Subbarayal, Sunil Chauhan, Reza Dana; Corneal Epithelial Pigment Epithelium Derived Factor is Reduced in a Murine Model of Dry Eye Disease. Invest. Ophthalmol. Vis. Sci. 2015;56(7 ):335.
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© ARVO (1962-2015); The Authors (2016-present)
Pigment epithelium derived factor (PEDF) has neurotrophic, anti-inflammatory and anti-angiogenic functions. Here, we aimed to determine whether corneal PEDF is reduced in dry eye disease (DED).
Dry eye disease was induced in 6-week-old female C57BL/6 mice for 7 and 14 days (n=5 each) by placing them in a controlled environment chamber. Control mice were kept in a normal environment (n=5). Corneas from normal and DED mice were immunohistochemically stained with a primary anti-PEDF antibody (goat) and a secondary anti-goat FITC antibody, and analyzed using fluorescent microscopy. A single cell suspension of corneal epithelial cells was prepared and analyzed for the expression of CD45 and PEDF using flow cytometry.
Immunohistochemistry showed decreased expression of PEDF in the corneal epithelium of DED mice at day 7 and 14 compared to normal mice. In addition, flow cytometry analysis showed that CD45- epithelial cells from DED mice have almost 50% less median fluorescence intensity for PEDF compared to corneal epithelial cells from normal mice.
Corneal epithelium derived PEDF is reduced in a murine model of DED suggesting its potential immunoregulatory role in the homeostasis of the ocular surface.
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